NudCD1, also known as CML66 or OVA66, is a protein initially identified as overexpressed in patients with chronic myelogenous leukemia. The mRNA of NudCD1 is expressed in heart and testis of normal tissues, and is overexpressed in several cancers. Previous studies have shown that the expression level of the protein correlates with tumoral phenotype, possibly interacting upstream of the Insulin Growth Factor - 1 Receptor (IGF-1R). The gene encoding the NudCD1 protein consists of 12 exons that can be alternative spliced, leading to the expression of three different isoforms. These isoforms possess a common region of 492 amino acids in their C-terminus region and have an isoform specific N-terminus. To determine the distinct function of each isoforms, we have localised the isoforms within the cells using immunofluorescence microscopy and used a quantitative proteomics approach (SILAC) to identify specific protein interaction partners for each isoforms. Localization studies showed a different subcellular distribution for the different isoforms, with the first isoform being nuclear, while the other two isoforms have distinct cytoplasmic and nuclear location. We found that the different NudCD1 isoforms have unique interacting partners, with the first isoform binding to a putative RNA helicase named DHX15 involved in mRNA splicing.

中文翻译：

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交替剪接的NudCD1亚型的蛋白质相互作用网络。

NudCD1，也称为CML66或OVA66，是一种最初被鉴定为在慢性粒细胞性白血病患者中过度表达的蛋白质。NudCD1的mRNA在正常组织的心脏和睾丸中表达，在几种癌症中过表达。先前的研究表明，该蛋白的表达水平与肿瘤表型相关，可能在胰岛素生长因子-1受体（IGF-1R）的上游相互作用。编码NudCD1蛋白的基因由12个外显子组成，这些外显子可以选择性剪接，导致表达三种不同的亚型。这些同工型在其C端区域共有492个氨基酸的共同区域，并具有同工型特异性N端。为了确定每种同工型的独特功能，我们已经使用免疫荧光显微镜在细胞内定位了同工型，并使用了定量蛋白质组学方法（SILAC）来识别每种同工型的特定蛋白质相互作用伴侣。本地化研究显示不同同工型的亚细胞分布不同，第一个同工型是核型，而其他两个同工型则具有不同的胞质和核位置。我们发现不同的NudCD1同工型具有独特的相互作用伙伴，第一个同工型与假定的RNA解旋酶DHX15结合，参与了mRNA的剪接。而其他两个同工型则具有明显的胞质和核位置。我们发现不同的NudCD1同工型具有独特的相互作用伙伴，第一个同工型与假定的RNA解旋酶DHX15结合，参与了mRNA的剪接。而其他两个同工型则具有明显的胞质和核位置。我们发现不同的NudCD1同工型具有独特的相互作用伙伴，第一个同工型与假定的RNA解旋酶DHX15结合，参与了mRNA的剪接。