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Multiplexed Electrochemical Detection of MiRNAs from Sera of Glioma Patients at Different Stages via the Novel Conjugates of Conducting Magnetic Microbeads and Diblock Oligonucleotide-Modified Gold Nanoparticles
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-10-05 00:00:00 , DOI: 10.1021/acs.analchem.7b02342 Jingrui Wang 1 , Zhixuan Lu 1 , Hailin Tang 2 , Ling Wu 1 , Zixiao Wang 1 , Minghua Wu 3 , Xinyao Yi 1 , Jianxiu Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-10-05 00:00:00 , DOI: 10.1021/acs.analchem.7b02342 Jingrui Wang 1 , Zhixuan Lu 1 , Hailin Tang 2 , Ling Wu 1 , Zixiao Wang 1 , Minghua Wu 3 , Xinyao Yi 1 , Jianxiu Wang 1
Affiliation
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MicroRNAs (miRNAs) serve as diagnostic and prognostic biomarkers for a wide variety of cancers. Via the novel conjugates of gold nanoparticle-coated magnetic microbeads (AuNP-MMBs) and the diblock oligonucleotide (ODN)-modified AuNPs, multiplexed electrochemical assay of miRNAs was performed. The hybridization to target miRNAs leads to the conformational change of the hairpin-structured ODN probes, and the attachment of the diblock ODN-modified AuNPs was achieved. By examining the oxidation peak currents of methylene blue (MB) and ferrocene (Fc) moieties residing on the diblock ODNs, simultaneous quantification of miRNA-182 and miRNA-381 was conducted. The detection signals were significantly enhanced due to the numerous MB and Fc tags on the AuNPs. The proposed assay was highly selective for discriminating miRNAs with similar sequences, and detection limits of 0.20 fM and 0.12 fM for miRNA-182 and miRNA-381, respectively, were achieved. The feasibility of the method for sensitive determination of miRNA-182 and miRNA-381 from serum samples of glioma patients at different stages was demonstrated. The sensing protocol thus holds great potential for early diagnosis and treatment of cancer patients.
中文翻译：
通过新型的导电磁珠和双嵌段寡核苷酸修饰的金纳米粒子偶联物，对胶质瘤患者血清中不同阶段的miRNA进行多重电化学检测。
MicroRNA（miRNA）作为多种癌症的诊断和预后生物标志物。通过金纳米颗粒包覆的磁性微珠（AuNP-MMBs）和双嵌段寡核苷酸（ODN）修饰的AuNPs的新型共轭物，对miRNA进行了多重电化学检测。与靶标miRNA的杂交导致发夹结构ODN探针的构象发生变化，并实现了对二嵌段ODN修饰的AuNP的附着。通过检查位于二嵌段ODN上的亚甲基蓝（MB）和二茂铁（Fc）部分的氧化峰值电流，对miRNA-182和miRNA-381进行了同时定量。由于AuNP上有许多MB和Fc标签，检测信号得到了显着增强。拟议的测定法对区分具有相似序列的miRNA具有高度选择性，miRNA-182和miRNA-381的检出限分别为0.20 fM和0.12 fM。证明了该方法在不同阶段从胶质瘤患者血清中敏感测定miRNA-182和miRNA-381的可行性。因此，该传感方案在癌症患者的早期诊断和治疗方面具有巨大的潜力。
更新日期：2017-10-05
中文翻译：
通过新型的导电磁珠和双嵌段寡核苷酸修饰的金纳米粒子偶联物，对胶质瘤患者血清中不同阶段的miRNA进行多重电化学检测。
MicroRNA（miRNA）作为多种癌症的诊断和预后生物标志物。通过金纳米颗粒包覆的磁性微珠（AuNP-MMBs）和双嵌段寡核苷酸（ODN）修饰的AuNPs的新型共轭物，对miRNA进行了多重电化学检测。与靶标miRNA的杂交导致发夹结构ODN探针的构象发生变化，并实现了对二嵌段ODN修饰的AuNP的附着。通过检查位于二嵌段ODN上的亚甲基蓝（MB）和二茂铁（Fc）部分的氧化峰值电流，对miRNA-182和miRNA-381进行了同时定量。由于AuNP上有许多MB和Fc标签，检测信号得到了显着增强。拟议的测定法对区分具有相似序列的miRNA具有高度选择性，miRNA-182和miRNA-381的检出限分别为0.20 fM和0.12 fM。证明了该方法在不同阶段从胶质瘤患者血清中敏感测定miRNA-182和miRNA-381的可行性。因此，该传感方案在癌症患者的早期诊断和治疗方面具有巨大的潜力。
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