A new NADH-dependent carbonyl reductase RhCR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl (S)-8-chloro-6-hydroxyoctanoate [(S)-ECHO], an important chiral precursor for the synthesis of (R)-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing RhCR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L⁻¹ (2 M) of ECOO were stoichiometrically converted to (S)-ECHO in a space–time yield of 1580 g L⁻¹ d⁻¹ without the external addition of any expensive cofactor.

中文翻译：

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通过新发现的还原酶Rh CR高效合成时空产率为1580 g L ^-1  d ^-1的ε-羟基酯

一种新的NADH依赖性羰基还原酶的Rh CR能够有效地降低ε酮酯乙基-8-氯-6- oxooctanoate（ECOO），得到乙基（小号）-8-氯-6-羟基辛[（小号）-echo]对于（合成中的手性重要前体- [R）-α-α-硫辛酸，从识别的红球菌属。ECU1014。使用重组大肠杆菌表达细胞的Rh CR和葡萄糖脱氢酶用于辅因子的再生和440克L- ^-1（2 M）ECOO被化学计量转化成（小号）-echo在1580克L-一个时空产率^-1  d ^-1 无需在外部添加任何昂贵的辅因子。