血小板浓缩液对人体血小板的磁性纳米颗粒标记，用于回收和存活研究,ACS Applied Materials & Interfaces

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血小板浓缩液对人体血小板的磁性纳米颗粒标记，用于回收和存活研究
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-09-29 00:00:00 , DOI: 10.1021/acsami.7b10113
Konstanze Aurich ₁ , Jan Wesche ₁ , Raghavendra Palankar ₁ , Rabea Schlüter ₂ , Tamam Bakchoul ₁ , Andreas Greinacher ₁

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血小板是最小的血细胞，对止血很重要。浓缩血小板（PC）是输注的预防或治疗出血的药物。通常，通过体外测试评估PC中的血小板功能。然而，非常需要对这些血小板进行体内测试。为了在临床研究范围内将PC患者输血后的血小板与患者或先证者自身细胞区分开来，需要在输血前以最小的预激活有效地标记血小板。在这里，我们报告了一种通过用人血清白蛋白（HSA）修饰纳米粒子壳来改善Resovist（一种FDA批准的MRI造影剂）中所含的Ferucarbotran磁性纳米粒子的细胞摄取的方法。HSA-ferucarbotran纳米颗粒和磁性标记的血小板均根据EU-GMP指南生产。标记后的血小板功能通过透光聚集法和确定作为血小板活化标志物的CD62P的表达来评估。磁性标记不会损害血小板功能，并且血小板对激动剂显示出合理的活化反应。在NOD / SCID小鼠中进行的血小板生存研究得出了磁性标记和未标记的血小板可比的生存行为。另外，可以通过磁分离从全血中回收标记的血小板。磁性标记不会损害血小板功能，并且血小板对激动剂显示出合理的活化反应。在NOD / SCID小鼠中进行的血小板生存研究得出了磁性标记和未标记血小板的可比生存行为。另外，可以通过磁分离从全血中回收标记的血小板。磁性标记不会损害血小板功能，并且血小板对激动剂显示出合理的活化反应。在NOD / SCID小鼠中进行的血小板生存研究得出了磁性标记和未标记的血小板可比的生存行为。另外，可以通过磁分离从全血中回收标记的血小板。

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Magnetic Nanoparticle Labeling of Human Platelets from Platelet Concentrates for Recovery and Survival Studies

Platelets are the smallest blood cells and important for hemostasis. Platelet concentrates (PC) are medicinal products transfused to prevent or treat bleeding. Typically, platelets in PCs are assessed by in vitro tests for their function. However, in vivo testing of these platelets is highly desirable. To distinguish transfused platelets from patients or probands own cells after PC transfusions within the scope of clinical studies, platelets need to be efficiently labeled with minimal preactivation prior to transfusion. Here we report on a method for improved cell uptake of ferucarbotran magnetic nanoparticles contained in Resovist, an FDA-approved MRI contrast agent, by modifying the nanoparticle shell with human serum albumin (HSA). Both HSA-ferucarbotran nanoparticles and magnetically labeled platelets were produced according to EU-GMP guidelines. Platelet function after labeling was evaluated by light transmission aggregometry and by determination of expression of CD62P as platelet activation marker. Magnetic labeling does not impair platelet function and platelets showed reasonable activation response to agonists. Platelet survival studies in NOD/SCID-mice resulted in comparable survival behavior of magnetically labeled and nonlabeled platelets. Additionally, labeled platelets can be recovered from whole blood by magnetic separation.

更新日期：2017-09-30

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