用于细胞捕获和释放的光响应 Janus 粒子涂层,ACS Macro Letters

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用于细胞捕获和释放的光响应 Janus 粒子涂层
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-09-27 00:00:00 , DOI: 10.1021/acsmacrolett.7b00714
Ziquan Cao _{1,

2} , Qing Bian ₁ , Ying Chen ₂ , Fuxin Liang ₂ , Guojie Wang ₁

Affiliation

实现了基于 Janus 复合颗粒的坚固光响应涂层。首先，草莓状二氧化硅 Janus 颗粒是通过溶胶-凝胶工艺在不完整的乳液界面合成的。二氧化硅Janus颗粒的一侧具有纳米级粗糙度，另一侧是平坦的。然后，将含有螺吡喃的聚合物刷接枝到所合成的 Janus 颗粒的粗糙半球形一侧，另一平面用咪唑啉基团修饰。当Janus复合粒子在环氧树脂基材表面自组织成一层时，光响应聚合物刷端粗糙的半球形侧面指向空气。咪唑啉基团与环氧树脂中的环氧基团反应形成坚固的智能涂层。通过紫外和可见光照射，涂层可以在疏水和亲水之间可逆地触发，这归因于螺吡喃部分的异构化。当疏水性闭环螺吡喃形式突出时，HeLa细胞可以有效地被捕获到涂层上。紫外光照射后，闭环螺吡喃形式变为亲水性开环两性离子部花青形式，然后释放捕获的细胞。这项工作显示出设计先进的智能生物界面的巨大潜力。闭环螺吡喃形式变为亲水开环两性离子部花青形式，然后释放捕获的细胞。这项工作显示出设计先进的智能生物界面的巨大潜力。闭环螺吡喃形式变为亲水开环两性离子部花青形式，然后释放捕获的细胞。这项工作显示出设计先进的智能生物界面的巨大潜力。

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Light-Responsive Janus-Particle-Based Coatings for Cell Capture and Release

A robust light-responsive coating based on Janus composite particles is achieved. First, strawberry-like silica Janus particles are synthesized by the sol–gel process at a patchy emulsion interface. One side of the silica Janus particles possesses nanoscale roughness, and the other side is flat. Then, spiropyran-containing polymer brushes are grafted onto the coarse hemispherical side of the as-synthesized Janus particles, and the other flat side is modified with imidazoline groups. The light-responsive polymer brush-terminated coarse hemispherical sides direct toward the air when the Janus composite particles self-organize into a layer on the surface of epoxy resin substrate. The imidazoline groups react with the epoxy groups in the epoxy resin to form a robust smart coating. The coating can be reversibly triggered between hydrophobic and hydrophilic by UV and visible-light irradiation, which is attributed to the isomerization of spiropyran moieties. When the hydrophobic ring-closed spiropyran form is prominent, HeLa cells can be effectively captured onto the coating. After UV light irradiation, the ring-closed spiropyran form changes to the hydrophilic ring-opened zwitterionic merocyanine form, and then the captured cells are released. This work shows promising potential for engineering advanced smart biointerfaces.

更新日期：2017-09-27

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