Protein-Mediated Colloidal Assembly,Journal of the American Chemical Society

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Protein-Mediated Colloidal Assembly
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-09-27 00:00:00 , DOI: 10.1021/jacs.7b07798
Maiko Obana ₁ , Bradley R. Silverman ₁ , David A. Tirrell ₁

Affiliation

Programmable colloidal assembly enables the creation of mesoscale materials in a bottom-up manner. Although DNA oligonucleotides have been used extensively as the programmable units in this paradigm, proteins, which exhibit more diverse modes of association and function, have not been widely used to direct colloidal assembly. Here we use protein–protein interactions to drive controlled aggregation of polystyrene microparticles, either through reversible coiled-coil interactions or through intermolecular isopeptide linkages. The sizes of the resulting aggregates are tunable and can be controlled by the concentration of immobilized surface proteins. Moreover, particles coated with different protein pairs undergo orthogonal assembly. We demonstrate that aggregates formed by association of coiled-coil proteins, in contrast to those linked by isopeptide bonds, are dispersed by treatment with chemical denaturants or soluble competing proteins. Finally, we show that protein–protein interactions can be used to assemble complex core–shell aggregates. This work illustrates a versatile strategy for engineering colloidal systems for use in materials science and biotechnology.

中文翻译：

蛋白质介导的胶体组装

可编程的胶体组装能够以自下而上的方式创建中尺度材料。尽管DNA寡核苷酸已被广泛用作该范例中的可编程单元，但显示出更多种缔合和功能模式的蛋白质尚未广泛用于指导胶体组装。在这里，我们使用蛋白质-蛋白质相互作用，通过可逆的卷曲螺旋相互作用或分子间异肽键合来驱动聚苯乙烯微粒的受控聚集。所得聚集体的大小是可调的，可以通过固定的表面蛋白的浓度进行控制。而且，涂覆有不同蛋白质对的颗粒经历正交组装。我们证明了由卷曲螺旋蛋白缔合形成的聚集体，与通过异肽键连接的那些相反，通过用化学变性剂或可溶性竞争性蛋白质处理而分散。最后，我们证明了蛋白质-蛋白质相互作用可用于组装复杂的核-壳聚集体。这项工作说明了用于材料科学和生物技术的工程胶体系统的通用策略。

更新日期：2017-09-28

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