1,2,4-丁三醇的生物技术生产：从可再生生物质合成含能材料前体的有效方法。,Scientific Reports

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1,2,4-丁三醇的生物技术生产：从可再生生物质合成含能材料前体的有效方法。
Scientific Reports ( IF 3.8 ) Pub Date : 2015-Dec-16 , DOI: 10.1038/srep18149
Yujin Cao , Wei Niu , Jiantao Guo , Mo Xian , Huizhou Liu

1,2,4-丁三醇（BT）是一种有价值的化学品，在许多不同领域中都有广泛的应用。合成BT的传统化学路线存在许多缺点，例如苛刻的反应条件，多个步骤和较差的选择性，限制了其工业生产。在这项研究中，构建了一种工程化的大肠杆菌菌株以从木糖生产BT，木糖是木质纤维素生物质的主要成分。通过共表达来自新月形芽孢杆菌的木糖脱氢酶（CCxylB）和木糖内酯酶（xylC），天然大肠杆菌木糖酸脱水酶（yjhG），恶臭假单胞菌的2-酮酸脱羧酶（mdlC）和天然大肠杆菌醛还原酶（xdl） adhP）在大肠杆菌BL21 star（DE3）中，该重组菌株可有效地将木糖转化为BT。此外，通过破坏编码木糖异构酶和木糖酶的两个基因（xylA和EcxylB），阻断了负责大肠杆菌中木糖代谢的竞争途径。在补料分批条件下，工程菌株BL21ΔxylAB/ pE-mdlCxylBC＆pA-adhPyjhG从20 g / L的木糖中产生高达3.92 g / L的BT，相当于摩尔产率为27.7％。这些结果表明，工程化的大肠杆菌对于大规模生产BT具有广阔的前景。

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Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass.

1,2,4-Butanetriol (BT) is a valuable chemical with extensive applications in many different fields. The traditional chemical routes to synthesize BT suffer from many drawbacks, e.g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed to produce BT from xylose, which is a major component of the lignocellulosic biomass. Through the coexpression of a xylose dehydrogenase (CCxylB) and a xylonolactonase (xylC) from Caulobacter crescentus, native E. coli xylonate dehydratase (yjhG), a 2-keto acid decarboxylase from Pseudomonas putida (mdlC) and native E. coli aldehyde reductase (adhP) in E. coli BL21 star(DE3), the recombinant strain could efficiently convert xylose to BT. Furthermore, the competitive pathway responsible for xylose metabolism in E. coli was blocked by disrupting two genes (xylA and EcxylB) encoding xylose isomerase and xyloluse kinase. Under fed-batch conditions, the engineered strain BL21ΔxylAB/pE-mdlCxylBC&pA-adhPyjhG produced up to 3.92 g/L of BT from 20 g/L of xylose, corresponding to a molar yield of 27.7%. These results suggest that the engineered E. coli has a promising prospect for the large-scale production of BT.

更新日期：2017-01-31

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