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Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-09-26 00:00:00 , DOI: 10.1021/acs.biomac.7b00568 Jisoo Shin 1 , Eun Jung Choi 2 , Jung Ho Cho 1 , Ann-Na Cho 1 , Yoonhee Jin 1 , Kisuk Yang 1 , Changsik Song 2 , Seung-Woo Cho 1
Biomacromolecules ( IF 5.5 ) Pub Date : 2017-09-26 00:00:00 , DOI: 10.1021/acs.biomac.7b00568 Jisoo Shin 1 , Eun Jung Choi 2 , Jung Ho Cho 1 , Ann-Na Cho 1 , Yoonhee Jin 1 , Kisuk Yang 1 , Changsik Song 2 , Seung-Woo Cho 1
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Electrically conductive hyaluronic acid (HA) hydrogels incorporated with single-walled carbon nanotubes (CNTs) and/or polypyrrole (PPy) were developed to promote differentiation of human neural stem/progenitor cells (hNSPCs). The CNT and PPy nanocomposites, which do not easily disperse in aqueous phases, dispersed well and were efficiently incorporated into catechol-functionalized HA (HA–CA) hydrogels by the oxidative catechol chemistry used for hydrogel cross-linking. The prepared electroconductive HA hydrogels provided dynamic, electrically conductive three-dimensional (3D) extracellular matrix environments that were biocompatible with hNSPCs. The HA–CA hydrogels containing CNT and/or PPy significantly promoted neuronal differentiation of human fetal neural stem cells (hfNSCs) and human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) with improved electrophysiological functionality when compared to differentiation of these cells in a bare HA–CA hydrogel without electroconductive motifs. Calcium channel expression was upregulated, depolarization was activated, and intracellular calcium influx was increased in hNSPCs that were differentiated in 3D electroconductive HA–CA hydrogels; these data suggest a potential mechanism for stem cell neurogenesis. Overall, our bioinspired, electroconductive HA hydrogels provide a promising cell-culture platform and tissue-engineering scaffold to improve neuronal regeneration.
中文翻译：
邻苯二酚介导的分散与碳纳米管和聚吡咯的三维导电透明质酸水凝胶增强人神经干细胞的神经发生。
开发了与单壁碳纳米管（CNT）和/或聚吡咯（PPy）结合的导电透明质酸（HA）水凝胶，以促进人类神经干/祖细胞（hNSPC）的分化。CNT和PPy纳米复合材料不易分散在水相中，分散得很好，并通过用于水凝胶交联的氧化邻苯二酚化学方法有效地掺入了邻苯二酚官能化的HA（HA–CA）水凝胶中。制备的导电HA水凝胶提供了与hNSPC具有生物相容性的动态，导电的三维（3D）细胞外基质环境。含有CNT和/或PPy的HA–CA水凝胶可显着促进人类胎儿神经干细胞（hfNSCs）和人类诱导的多能干细胞源性神经祖细胞（hiPSC-NPCs）的神经元分化，与之相比，它们具有更好的电生理功能裸露的HA-CA水凝胶中的细胞没有导电基序。在3D导电HA–CA水凝胶中分化的hNSPC中，钙通道表达被上调，去极化被激活，并且细胞内钙内流增加。这些数据表明干细胞神经发生的潜在机制。总体而言，我们受生物启发的导电HA水凝胶提供了有希望的细胞培养平台和组织工程支架，可改善神经元的再生。
更新日期：2017-09-26
中文翻译：
邻苯二酚介导的分散与碳纳米管和聚吡咯的三维导电透明质酸水凝胶增强人神经干细胞的神经发生。
开发了与单壁碳纳米管（CNT）和/或聚吡咯（PPy）结合的导电透明质酸（HA）水凝胶，以促进人类神经干/祖细胞（hNSPC）的分化。CNT和PPy纳米复合材料不易分散在水相中，分散得很好，并通过用于水凝胶交联的氧化邻苯二酚化学方法有效地掺入了邻苯二酚官能化的HA（HA–CA）水凝胶中。制备的导电HA水凝胶提供了与hNSPC具有生物相容性的动态，导电的三维（3D）细胞外基质环境。含有CNT和/或PPy的HA–CA水凝胶可显着促进人类胎儿神经干细胞（hfNSCs）和人类诱导的多能干细胞源性神经祖细胞（hiPSC-NPCs）的神经元分化，与之相比，它们具有更好的电生理功能裸露的HA-CA水凝胶中的细胞没有导电基序。在3D导电HA–CA水凝胶中分化的hNSPC中，钙通道表达被上调，去极化被激活，并且细胞内钙内流增加。这些数据表明干细胞神经发生的潜在机制。总体而言，我们受生物启发的导电HA水凝胶提供了有希望的细胞培养平台和组织工程支架，可改善神经元的再生。
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