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A Printed Multicomponent Paper Sensor for Bacterial Detection.
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Sep-26 , DOI: 10.1038/s41598-017-12549-3 M Monsur Ali 1 , Christine L Brown 2 , Sana Jahanshahi-Anbuhi 2 , Balamurali Kannan 1 , Yingfu Li 3 , Carlos D M Filipe 2 , John D Brennan 1
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Sep-26 , DOI: 10.1038/s41598-017-12549-3 M Monsur Ali 1 , Christine L Brown 2 , Sana Jahanshahi-Anbuhi 2 , Balamurali Kannan 1 , Yingfu Li 3 , Carlos D M Filipe 2 , John D Brennan 1
Affiliation
We present a simple all-in-one paper-based sensor for E. coli detection using a composite ink made of a fluorogenic DNAzyme probe for bacterial recognition and signal generation, lysozyme that lyses whole bacterial cells, and pullulan/trehalose sugars that stabilize printed bioactive molecules. The paper sensor is capable of producing a fluorescence signal as a readout within 5 minutes upon contacting E. coli, can achieve a limit of detection of 100 cells/mL, in a variety of sample matrixes, without sample enrichment, and remains stable for at least 6 months when stored at ambient temperature. Therefore, this simple paper sensor provides rapid bacterial testing on site, and can be shipped and stored under ambient conditions to benefit users living in resource-limited regions.
中文翻译:
用于细菌检测的印刷多组分纸张传感器。
我们提出了一种简单的一体式纸基传感器,用于大肠杆菌检测,使用复合墨水,该复合墨水由用于细菌识别和信号生成的荧光 DNAzyme 探针、裂解整个细菌细胞的溶菌酶以及稳定印刷的支链淀粉/海藻糖制成。生物活性分子。纸质传感器能够在接触大肠杆菌后 5 分钟内产生荧光信号作为读数,在各种样品基质中可以达到 100 个细胞/mL 的检测限,无需样品富集,并且在常温下保存至少6个月。因此,这种简单的纸质传感器可以在现场进行快速细菌检测,并且可以在常温条件下运输和储存,以造福生活在资源有限地区的用户。
更新日期:2017-09-26
中文翻译:
用于细菌检测的印刷多组分纸张传感器。
我们提出了一种简单的一体式纸基传感器,用于大肠杆菌检测,使用复合墨水,该复合墨水由用于细菌识别和信号生成的荧光 DNAzyme 探针、裂解整个细菌细胞的溶菌酶以及稳定印刷的支链淀粉/海藻糖制成。生物活性分子。纸质传感器能够在接触大肠杆菌后 5 分钟内产生荧光信号作为读数,在各种样品基质中可以达到 100 个细胞/mL 的检测限,无需样品富集,并且在常温下保存至少6个月。因此,这种简单的纸质传感器可以在现场进行快速细菌检测,并且可以在常温条件下运输和储存,以造福生活在资源有限地区的用户。