用于生物医学应用的包含 DNA 折纸、金纳米棒和抗癌药物的磷酸钙纳米复合材料的制备,Advanced Healthcare Materials

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用于生物医学应用的包含 DNA 折纸、金纳米棒和抗癌药物的磷酸钙纳米复合材料的制备
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-09-22 , DOI: 10.1002/adhm.201700664
Hongbo Zhang _{1,

2,

3} , Xiangmeng Qu _{1,

2,

4} , Hong Chen ₅ , Haixin Kong ₁ , Ruihua Ding ₂ , Dong Chen ₂ , Xu Zhang ₂ , Hao Pei ₄ , Hélder A Santos ₆ , Mingtan Hai _{1,

2} , David A Weitz ₂

Affiliation

DNA折纸是通过在任意控制下在纳米级折叠DNA链来设计的。由于其固有的生物学性质，DNA 折纸被用于药物递送以增强协同作用和多药耐药抑制、癌症诊断和许多其他生物医学应用，在这些方面显示出巨大的潜力。然而，DNA折纸固有的不稳定性和低有效载荷能力限制了其生物医学应用。在这里，本文报道了一种先进的生物相容性纳米复合纳米复合材料的制造，该复合材料可保护 DNA 折纸免于降解和药物负载。将 DNA 折纸、金纳米棒和分子靶向药物结合到 pH 响应性磷酸钙 [Ca ₃ (PO ₄ ) ₂] 纳米粒子。随后，将一层薄薄的磷脂涂覆在 Ca ₃ (PO ₄ ) ₂纳米颗粒上，以提供更好的生物相容性。所制备的纳米复合材料显示出高载药量、良好的生物相容性以及光热和 pH 响应的有效载荷释放曲线，并充分保护 DNA 折纸免于降解。DNA折纸与抗癌药物的共递送协同诱导癌细胞凋亡，降低多药耐药性，提高对人表皮生长因子受体2阳性细胞的靶向杀伤效率。预计这种纳米复合材料将为各种临床和生物医学应用开辟新的视野。

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Fabrication of Calcium Phosphate‐Based Nanocomposites Incorporating DNA Origami, Gold Nanorods, and Anticancer Drugs for Biomedical Applications

DNA origami is designed by folding DNA strands at the nanoscale with arbitrary control. Due to its inherent biological nature, DNA origami is used in drug delivery for enhancement of synergism and multidrug resistance inhibition, cancer diagnosis, and many other biomedical applications, where it shows great potential. However, the inherent instability and low payload capacity of DNA origami restrict its biomedical applications. Here, this paper reports the fabrication of an advanced biocompatible nano‐in‐nanocomposite, which protects DNA origami from degradation and facilities drug loading. The DNA origami, gold nanorods, and molecular targeted drugs are co‐incorporated into pH responsive calcium phosphate [Ca₃(PO₄)₂] nanoparticles. Subsequently, a thin layer of phospholipid is coated onto the Ca₃(PO₄)₂ nanoparticle to offer better biocompatibility. The fabricated nanocomposite shows high drug loading capacity, good biocompatibility, and a photothermal and pH‐responsive payload release profile and it fully protects DNA origami from degradation. The codelivery of DNA origami with cancer drugs synergistically induces cancer cell apoptosis, reduces the multidrug resistance, and enhances the targeted killing efficiency toward human epidermal growth factor receptor 2 positive cells. This nanocomposite is foreseen to open new horizons for a variety of clinical and biomedical applications.

更新日期：2017-09-22

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