To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal–organic framework (MOF), NH₂-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF–bacteriophage biosensor based on the photoluminescence quenching phenomena: limit of detection (31 CFU/mL) and range of detection (40 to 4 × 10⁸ CFU/mL). This is the first report exploiting the use of an MOF–bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity, regenerability, extended range of linear detection, and stability for long-term storage (even at room temperature).

中文翻译：

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MOF –噬菌体生物传感器，用于高灵敏度和特异性检测金黄色葡萄球菌

为了生产一种针对金黄色葡萄球菌的灵敏且特异的生物传感器，噬菌体已与水可分散且环境稳定的金属有机框架（MOF）NH ₂ -MIL-53（Fe）结合。通过使用戊二醛作为交联剂已经实现了MOF与噬菌体的缀合。拟议的MOF噬菌体生物传感器基于光致发光猝灭现象，实现了合成样品和实际样品中金黄色葡萄球菌的高灵敏度检测：检测限（31 CFU / mL）和检测范围（40至4×10 ⁸ CFU） / mL）。这是第一个利用MOF-噬菌体复合物对金黄色葡萄球菌进行生物传感的报告。。我们的研究结果表明，提出的生物传感器比大多数以前的方法更敏感，同时具有一些先进的功能，如特异性，可再生性，线性检测的扩展范围以及长期保存（即使在室温下）的稳定性。