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[Determination of sennosides and degraded products in the process of sennoside metabolism by HPLC].
Se pu = Chinese journal of chromatography/ Zhongguo hua xue hui. Pub Date : 2004 Jan
Sun, Yan, Li, Xuetuo, Yu, Xingju
Se pu = Chinese journal of chromatography/ Zhongguo hua xue hui. Pub Date : 2004 Jan
Sun, Yan, Li, Xuetuo, Yu, Xingju
A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C18 column (250 mm x 4.6 mm i.d., 10 microm); column temperature, 40 degrees C; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100% A --> (20 min) 100% B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.
中文翻译:
高效液相色谱法测定sennoside代谢过程中的sennosides及其降解产物
开发了一种通过线性梯度高效液相色谱法分离测定森诺糖苷降解产物中的森诺糖苷A和B以及主要成分(森尼丁A和B)的方法。分离条件如下:柱,Spherisorb C18柱(250mm×4.6mm,10μm)。柱温40℃;检测波长360 nm;流动相A,1.25%乙酸水溶液;流动相B,甲醇; 线性梯度,100%A->(20分钟)100%B.该方法有效,快速,准确且可重现。令人满意的结果表明,该新方法作为一种实时分析山参皂苷代谢过程的方法,具有一定的实用价值。
更新日期:2017-01-31
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
高效液相色谱法测定sennoside代谢过程中的sennosides及其降解产物
开发了一种通过线性梯度高效液相色谱法分离测定森诺糖苷降解产物中的森诺糖苷A和B以及主要成分(森尼丁A和B)的方法。分离条件如下:柱,Spherisorb C18柱(250mm×4.6mm,10μm)。柱温40℃;检测波长360 nm;流动相A,1.25%乙酸水溶液;流动相B,甲醇; 线性梯度,100%A->(20分钟)100%B.该方法有效,快速,准确且可重现。令人满意的结果表明,该新方法作为一种实时分析山参皂苷代谢过程的方法,具有一定的实用价值。