Coupling Bioflocculation of Dehalococcoides mccartyi to High-Rate Reductive Dehalogenation of Chlorinated Ethenes,Environmental Science & Technology

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Coupling Bioflocculation of Dehalococcoides mccartyi to High-Rate Reductive Dehalogenation of Chlorinated Ethenes
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-09-15 00:00:00 , DOI: 10.1021/acs.est.7b03097
Anca G. Delgado _{1,

2} , Devyn Fajardo-Williams _{1,

2} , Emily Bondank ₁ , Sofia Esquivel-Elizondo _{1,

2} , Rosa Krajmalnik-Brown _{1,

2}

Affiliation

Continuous bioreactors operated at low hydraulic retention times have rarely been explored for reductive dehalogenation of chlorinated ethenes. The inability to consistently develop such bioreactors affects the way growth approaches for Dehalococcoides mccartyi bioaugmentation cultures are envisioned. It also affects interpretation of results from in situ continuous treatment processes. We report bioreactor performance and dehalogenation kinetics of a D. mccartyi-containing consortium in an upflow bioreactor. When fed synthetic groundwater at 11–3.6 h HRT, the upflow bioreactor removed >99.7% of the influent trichloroethene (1.5–2.8 mM) and produced ethene as the main product. A trichloroethene removal rate of 98.51 ± 0.05 me^– equiv L^–1 d^–1 was achieved at 3.6 h HRT. D. mccartyi cell densities were 10¹³ and 10¹² 16S rRNA gene copies L^–1 in the bioflocs and planktonic culture, respectively. When challenged with a feed of natural groundwater containing various competing electron acceptors and 0.3–0.4 mM trichloroethene, trichloroethene removal was sustained at >99.6%. Electron micrographs revealed that D. mccartyi were abundant within the bioflocs, not only in multispecies structures, but also as self-aggregated microcolonies. This study provides fundamental evidence toward the feasibility of upflow bioreactors containing D. mccartyi as high-density culture production tools or as a high-rate, real-time remediation biotechnology.

中文翻译：

Dehalococcoides mccartyi的生物絮凝与氯化乙烯的高速率还原脱卤反应

在低水力停留时间下运行的连续生物反应器很少被探索用于氯化乙烯的还原脱卤。无法持续开发这样的生物反应器影响了设想的麦加脱球菌生物增强培养物的生长方法。它还会影响原位连续处理过程结果的解释。我们报告了D.麦卡蒂含财团在上流生物反应器中的生物反应器性能和脱卤动力学。当在HRT 11–3.6 h注入合成地下水时，上流生物反应器去除了进水三氯乙烯（1.5–2.8 mM）的99.7％以上，并产生了乙烯作为主要产物。三氯乙烯的去除率为98.51±0.05 me ^–当量L ^–1在3.6 h HRT达到d ^–1。D. mccartyi细胞密度在生物絮凝物和浮游培养物中分别为10 ¹³和10 ¹² 16S rRNA基因拷贝L ^–1。当使用含有各种竞争性电子受体和0.3–0.4 mM三氯乙烯的天然地下水进水时，三氯乙烯的去除率保持在> 99.6％。电子显微照片显示，麦考氏梭菌不仅在多物种结构中而且在自聚集的微菌落中都富集在生物絮凝物中。这项研究提供了基础的证据，证明含有麦卡迪氏菌的上流生物反应器的可行性作为高密度文化生产工具或作为高速率，实时修复生物技术。

更新日期：2017-09-15

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