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Genome sequencing reveals metabolic and cellular interdependence in an amoeba-kinetoplastid symbiosis.
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Sep-15 , DOI: 10.1038/s41598-017-11866-x
Goro Tanifuji , Ugo Cenci , Daniel Moog , Samuel Dean , Takuro Nakayama , Vojtěch David , Ivan Fiala , Bruce A. Curtis , Shannon J. Sibbald , Naoko T. Onodera , Morgan Colp , Pavel Flegontov , Jessica Johnson-MacKinnon , Michael McPhee , Yuji Inagaki , Tetsuo Hashimoto , Steven Kelly , Keith Gull , Julius Lukeš , John M. Archibald

Endosymbiotic relationships between eukaryotic and prokaryotic cells are common in nature. Endosymbioses between two eukaryotes are also known; cyanobacterium-derived plastids have spread horizontally when one eukaryote assimilated another. A unique instance of a non-photosynthetic, eukaryotic endosymbiont involves members of the genus Paramoeba, amoebozoans that infect marine animals such as farmed fish and sea urchins. Paramoeba species harbor endosymbionts belonging to the Kinetoplastea, a diverse group of flagellate protists including some that cause devastating diseases. To elucidate the nature of this eukaryote-eukaryote association, we sequenced the genomes and transcriptomes of Paramoeba pemaquidensis and its endosymbiont Perkinsela sp. The endosymbiont nuclear genome is ~9.5 Mbp in size, the smallest of a kinetoplastid thus far discovered. Genomic analyses show that Perkinsela sp. has lost the ability to make a flagellum but retains hallmark features of kinetoplastid biology, including polycistronic transcription, trans-splicing, and a glycosome-like organelle. Mosaic biochemical pathways suggest extensive 'cross-talk' between the two organisms, and electron microscopy shows that the endosymbiont ingests amoeba cytoplasm, a novel form of endosymbiont-host communication. Our data reveal the cell biological and biochemical basis of the obligate relationship between Perkinsela sp. and its amoeba host, and provide a foundation for understanding pathogenicity determinants in economically important Paramoeba.

中文翻译:

基因组测序揭示了变形虫-动素体共生中的代谢和细胞相互依赖性。

真核细胞与原核细胞之间的内共生关系在自然界很常见。还已知两种真核生物之间的内生共生酶。当一种真核生物同化另一种真核生物时,源自蓝细菌的质体水平扩散。非光合作用的真核内共生体的一个独特实例涉及拟南芥属的成员,变形虫感染了养殖鱼类和海胆等海洋动物。副粘虫物种具有属于动植物体的共生内生共生体,这是鞭毛状原生生物的不同种类,其中一些会导致毁灭性疾病。为了阐明这种真核生物-真核生物关联的性质,我们测序了Paramoeba pemaquidensis及其内共生菌Perkinsela sp的基因组和转录组。共生共生体核基因组的大小约为9.5 Mbp,是迄今为止发现的最小的动质体。基因组分析表明,Perkinsela sp。已经失去了制造鞭毛的能力,但保留了运动质体生物学的标志性特征,包括多顺反子转录,反式剪接和糖体样细胞器。马赛克生化途径表明两种生物之间存在广泛的“串扰”,电子显微镜显示内共生体摄取变形虫细胞质,这是内共生体与宿主之间交流的一种新形式。我们的数据揭示了Perkinsela sp。之间专心关系的细胞生物学和生化基础。及其变形虫宿主,并为了解具有重要经济意义的帕拉莫巴的致病性决定因素奠定了基础。包括多顺反子转录,反式剪接和糖体样细胞器。马赛克生化途径表明两种生物之间存在广泛的“串扰”,电子显微镜显示内共生体摄取变形虫细胞质,这是内共生体与宿主之间交流的一种新形式。我们的数据揭示了Perkinsela sp。之间专心关系的细胞生物学和生化基础。及其变形虫宿主,并为了解具有重要经济意义的帕拉莫巴的致病性决定因素奠定了基础。包括多顺反子转录,反式剪接和糖体样细胞器。马赛克生化途径表明两种生物之间存在广泛的“串扰”,电子显微镜显示内共生体摄取变形虫细胞质,这是内共生体与宿主之间交流的一种新形式。我们的数据揭示了Perkinsela sp。之间专心关系的细胞生物学和生化基础。及其变形虫宿主,并为了解具有重要经济意义的帕拉莫巴的致病性决定因素奠定了基础。我们的数据揭示了Perkinsela sp。之间专心关系的细胞生物学和生化基础。及其变形虫宿主,并为了解具有重要经济意义的帕拉莫巴的致病性决定因素奠定了基础。我们的数据揭示了Perkinsela sp。之间专心关系的细胞生物学和生化基础。及其变形虫宿主,并为了解具有重要经济意义的帕拉莫巴的致病性决定因素奠定了基础。
更新日期:2017-09-15
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