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Molecular Characterization of Extracts from Biorefinery Wastes and Evaluation of Their Plant Biostimulation
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-09-11 00:00:00 , DOI: 10.1021/acssuschemeng.7b01928 Davide Savy , Pierluigi Mazzei , Marios Drosos , Vincenza Cozzolino , Licia Lama 1 , Alessandro Piccolo
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-09-11 00:00:00 , DOI: 10.1021/acssuschemeng.7b01928 Davide Savy , Pierluigi Mazzei , Marios Drosos , Vincenza Cozzolino , Licia Lama 1 , Alessandro Piccolo
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Biorefinery residues from non-food biomasses are promising sources of sustainable agrochemicals. The molecular properties of water-soluble extracts from ligno-cellulosic biomass pretreated first by steam-explosion and then by enzymatic hydrolyses at different buffer doses, were assayed for bioactivity on maize. 13C and 31P nuclear magnetic resonance (NMR) spectra showed that extracts varied in phenolic and carboxyl content, while high performance size exclusion chromatography and diffusion ordered spectroscopy NMR revealed that Ox-BYP 1 obtained from wastes treated with a greater buffer dose contained small-sized molecules associated in apparently large metastable aggregates. Ox-BYP 2 separated from wastes treated with smaller buffer concentrations showed a more stable conformation. Both hydrolysates revealed a positive dose-dependent bioactivity toward maize growth. Ox-BYP 1 promoted plant fresh and dry weights and root length at 10 and 100 ppm but decreased seedling growth at 1 ppm. Instead, Ox-BYP 2 increased the whole plant growth at all assayed concentrations. Their different biostimulation effects were attributed to the toxicity of easily bioaccessible lignin-derived phenolics at small concentrations of Ox-BYP 1, which was removed by molecular self-assembly at greater concentrations. Conversely, the more strongly associated Ox-BYP 2 exerted a positive bioactivity even at small doses. The bioactivity of extracts from biorefinery wastes appeared to depend on molecular composition and, in turn, on waste pretreatments.
中文翻译：
生物炼制废料提取物的分子表征及其对植物生物刺激的评价
从非生物炼制残留-食品生物质是有希望的可持续农业化学品的来源。测定了木质纤维素生物质中水溶性提取物的分子特性，该分子提取物首先通过蒸汽爆炸然后通过不同缓冲剂量的酶促水解进行预处理，以测定其对玉米的生物活性。13 C和31P核磁共振（NMR）谱显示提取物的酚和羧基含量各不相同，而高性能尺寸排阻色谱和扩散有序光谱NMR显示，用更大缓冲剂量处理的废物中获得的Ox-BYP 1包含与分子相关的小分子显然是大的亚稳态聚集体。从以较小缓冲液浓度处理的废物中分离出的Ox-BYP 2显示出更稳定的构象。两种水解物均显示出对玉米生长呈正剂量依赖性的生物活性。Ox-BYP 1在10和100 ppm时促进了植物的鲜重和干重以及根长，但在1 ppm时降低了幼苗的生长。相反，在所有测定的浓度下，Ox-BYP 2都能促进整个植物的生长。它们不同的生物刺激作用归因于在低浓度的Ox-BYP 1下易于生物利用的木质素衍生的酚类化合物的毒性，在较高浓度下可通过分子自组装将其去除。相反，即使在小剂量时，结合更紧密的Ox-BYP 2仍具有积极的生物活性。来自生物精炼厂废物的提取物的生物活性似乎取决于分子组成，进而取决于废物的预处理。
更新日期：2017-09-11
中文翻译：
生物炼制废料提取物的分子表征及其对植物生物刺激的评价
从非生物炼制残留-食品生物质是有希望的可持续农业化学品的来源。测定了木质纤维素生物质中水溶性提取物的分子特性，该分子提取物首先通过蒸汽爆炸然后通过不同缓冲剂量的酶促水解进行预处理，以测定其对玉米的生物活性。13 C和31P核磁共振（NMR）谱显示提取物的酚和羧基含量各不相同，而高性能尺寸排阻色谱和扩散有序光谱NMR显示，用更大缓冲剂量处理的废物中获得的Ox-BYP 1包含与分子相关的小分子显然是大的亚稳态聚集体。从以较小缓冲液浓度处理的废物中分离出的Ox-BYP 2显示出更稳定的构象。两种水解物均显示出对玉米生长呈正剂量依赖性的生物活性。Ox-BYP 1在10和100 ppm时促进了植物的鲜重和干重以及根长，但在1 ppm时降低了幼苗的生长。相反，在所有测定的浓度下，Ox-BYP 2都能促进整个植物的生长。它们不同的生物刺激作用归因于在低浓度的Ox-BYP 1下易于生物利用的木质素衍生的酚类化合物的毒性，在较高浓度下可通过分子自组装将其去除。相反，即使在小剂量时，结合更紧密的Ox-BYP 2仍具有积极的生物活性。来自生物精炼厂废物的提取物的生物活性似乎取决于分子组成，进而取决于废物的预处理。
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