Development of simultaneous nitrification, denitrification and phosphorus removal (SNDPR) is a promising approach for domestic wastewater treatment. Mixed carbon sources by sodium acetate and glucose at various ratios (3:1, 1:1, and 1:3) were investigated to evaluate the effects on the sequencing batch reactors (SBR) based on aerobic granules. Results revealed that the mixed carbon did not change the settleability of aerobic granules, while glucose was more favorable for biomass retention induced by heterotrophs. Both carbon and ammonia nitrogen removal remained unaffected, while increased residual nitrate and phosphorus were detected with higher glucose ratios. Growing percentages of glucose led to more production of extracellular polymeric substances (EPS), especially polysaccharides (PS). Shifts of compositions of EPS were observed by the three-dimensional exaction and emission matrix (3D-EEM) fluorescence spectroscopy. MiSeq pyrosequencing technology demonstrated that the increasing ratios of glucose reduced the diversity of microbial community, though the dominant microbes remained unchanged. Phylogenetic classification of key groups involved in carbon, nitrogen and phosphorus removal suggested that mixed carbon source decidedly shaped the bacterial community.

同时硝化，反硝化和除磷（SNDPR）的开发是一种用于家庭废水处理的有前途的方法。研究了乙酸钠和葡萄糖以各种比例（3：1、1：1和1：3）混合的碳源，以评估对基于好氧颗粒的分批批处理反应器（SBR）的影响。结果表明，混合碳不会改变好氧颗粒的沉降性，而葡萄糖更有利于异养生物诱导生物量的保留。碳和氨氮的去除均不受影响，而较高的葡萄糖比率可检测到残留的硝酸盐和磷增加。葡萄糖百分比的增加导致细胞外聚合物（EPS），尤其是多糖（PS）的产生更多。通过三维精密度和发射矩阵（3D-EEM）荧光光谱观察到EPS组成的变化。MiSeq焦磷酸测序技术表明，尽管优势微生物保持不变，但葡萄糖比例的增加降低了微生物群落的多样性。碳，氮和磷去除的关键基团的系统发育分类表明，混合碳源决定性地形成了细菌群落。