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Stainless Steel Electrode for Sensitive Luminol Electrochemiluminescent Detection of H2O2, Glucose, and Glucose Oxidase Activity
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-08 00:00:00 , DOI: 10.1021/acs.analchem.7b01939 Shimeles Addisu Kitte 1, 2 , Wenyue Gao 1, 2 , Yuriy T. Zholudov 1, 3 , Liming Qi 1, 2 , Anaclet Nsabimana 1, 2 , Zhongyuan Liu 1 , Guobao Xu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-09-08 00:00:00 , DOI: 10.1021/acs.analchem.7b01939 Shimeles Addisu Kitte 1, 2 , Wenyue Gao 1, 2 , Yuriy T. Zholudov 1, 3 , Liming Qi 1, 2 , Anaclet Nsabimana 1, 2 , Zhongyuan Liu 1 , Guobao Xu 1
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Electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. Type 304 stainless steel electrode shows appealing ECL performance in the luminol–H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of 0.456 nM [signal-to-noise ratio (S/N) = 3]. The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective, and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, applications of stainless steel electrode-based ECL for detection of enzyme activities and enzyme substrates were further investigated by use of glucose oxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to ECL intensities over a range of 0.1–1000 μM and 0.001–0.7 units/mL with limits of detection of 0.076 μM and 0.00087 unit/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of their remarkable performance and user-friendly features, stainless steel electrodes hold great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.
中文翻译：
用于鲁米诺电化学发光检测H 2 O 2，葡萄糖和葡萄糖氧化酶活性的不锈钢电极
首次开发了一种不锈钢的电化学发光（ECL）应用，该材料是一种坚固且具有成本效益的材料。304型不锈钢电极在luminol–H 2 O 2系统中显示出引人注目的ECL性能。它可以检测H 2 O 2，线性范围为1至1000 nM，检测极限为0.456 nM [信噪比（S / N）= 3]。与以前报道的其他ECL方法相比，基于304型不锈钢电极的ECL方法更加灵敏，更具成本效益并且更简单。由于不锈钢电极具有出色的H 2 O 2检测和H 2 O 2检测性能参与许多重要的酶促反应，以葡萄糖氧化酶（GODx）和葡萄糖为代表的酶和底物，进一步研究了基于不锈钢电极的ECL在检测酶活性和酶底物方面的应用。在0.1–1000μM和0.001–0.7单位/ mL范围内，葡萄糖的浓度和GODx的活性与ECL强度成正比，检出限为0.076μM和0.00087单位/ mL（S / N = 3），分别。该方法已成功用于测定蜂蜜中的葡萄糖。由于其出色的性能和用户友好的特性，不锈钢电极在各种电分析应用中具有广阔的前景，例如生物传感，一次性传感器和可穿戴传感器。
更新日期：2017-09-08
中文翻译：
用于鲁米诺电化学发光检测H 2 O 2，葡萄糖和葡萄糖氧化酶活性的不锈钢电极
首次开发了一种不锈钢的电化学发光（ECL）应用，该材料是一种坚固且具有成本效益的材料。304型不锈钢电极在luminol–H 2 O 2系统中显示出引人注目的ECL性能。它可以检测H 2 O 2，线性范围为1至1000 nM，检测极限为0.456 nM [信噪比（S / N）= 3]。与以前报道的其他ECL方法相比，基于304型不锈钢电极的ECL方法更加灵敏，更具成本效益并且更简单。由于不锈钢电极具有出色的H 2 O 2检测和H 2 O 2检测性能参与许多重要的酶促反应，以葡萄糖氧化酶（GODx）和葡萄糖为代表的酶和底物，进一步研究了基于不锈钢电极的ECL在检测酶活性和酶底物方面的应用。在0.1–1000μM和0.001–0.7单位/ mL范围内，葡萄糖的浓度和GODx的活性与ECL强度成正比，检出限为0.076μM和0.00087单位/ mL（S / N = 3），分别。该方法已成功用于测定蜂蜜中的葡萄糖。由于其出色的性能和用户友好的特性，不锈钢电极在各种电分析应用中具有广阔的前景，例如生物传感，一次性传感器和可穿戴传感器。
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