基于罗丹明的荧光开启探针，用于线粒体中ATP的便捷传感和成像,ChemistrySelect

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基于罗丹明的荧光开启探针，用于线粒体中ATP的便捷传感和成像
ChemistrySelect ( IF 1.9 ) Pub Date : 2017-09-04 , DOI: 10.1002/slct.201701149
Omprakash Sunnapu _{1,

2} , Niranjan G. Kotla _{3,

4} , Balaji Maddiboyina ₅ , Srujan Marepally ₆ , Jeyabalan Shanmugapriya ₇ , Karuppannan Sekar ₂ , Subramanian Singaravadivel ₁ , Gandhi Sivaraman ₃

Affiliation

我们开发了一种“开启式”荧光探针ARP-1，作为通过氢键相互作用的比色和荧光化学传感器，用于5'-三磷酸腺苷（ATP）。该探针在10当量下显示出对ATP的“开启”荧光响应，并且荧光强度增强了15倍。已添加ATP。实验结果表明，ARP-1对ATP的响应行为与pH无关（pH 4.0-8.0）。新型化学传感器对来自其他核苷多磷酸盐（如ADP和AMP）的ATP具有很高的特异性。ATP的三磷酸单元与探针ARP-1的N原子之间的有利相互作用归因于氢键。因此，螺内酰胺开环是造成发射和肉眼变化增强的原因。从我们的发现很明显，链长以及NH，OH基团和磷酸基团的作用有助于探针和核苷酸之间的相互作用。在HeLa细胞中证明了细胞对ATP的通透性和选择性。共定位实验是用的MitoTracker绿和进行ARP-1示出的线粒体选择性成像能力ARP-1 。在HeLa细胞中进行的活细胞成像实验显示了具有荧光开启响应的ARP-1探针的高选择性。ARP-1 还可以探索了解细胞功能。

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Rhodamine‐Based Fluorescent Turn‐On Probe for Facile Sensing and Imaging of ATP in Mitochondria

We have developed a “turn‐on” fluorescent probe ARP‐1 as a colorimetric and fluorescent chemosensor for adenosine‐5’‐triphosphate (ATP) through hydrogen bond interactions. The probe exhibits “turn‐on” fluorescence response to ATP with a 15‐fold fluorescence intensity enhancement under 10 equiv. of ATP added. The experimental results show that the response behavior of ARP‐1 toward ATP is pH independent (pH 4.0‐8.0). The novel chemosensor has high specificity towards ATP from other nucleoside polyphosphates such as ADP and AMP. The favorable interaction between a triphosphate unit of ATP and N atoms of probe ARP‐1 is attributed to H‐bonding. Consequently, the enhanced emission and naked‐eye changes are attributed to spirolactam ring‐opening. It is evident from our findings that the role of the chain length as well as the NH, OH groups and the phosphate group(s) contribute to interaction between the probe and the nucleotide. Cell permeability and selectivity towards ATP was demonstrated in HeLa Cells. Colocalization experiments were carried out with MitoTracker green and ARP‐1 showing that the mitochondrion selective imaging ability of ARP‐1. The live cell imaging experiments in HeLa cells exhibited high selectivity of probe ARP‐1 with fluorescence turn‐On response. ARP‐1 could also be explored for understanding the cellular functions.

更新日期：2017-09-04

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