Treatment of full-thickness skin defects poses significant clinical challenges including risk of infection and severe scaring. Silver nanoparticle (NAg), an effective antimicrobial agent, has provided a promising therapeutic method for burn wounds. However, the detailed mechanism remains unknown. Hence, we constructed a metallic nanosilver particles-collagen/chitosan hybrid scaffold (NAg-CCS) and investigated its potential effects on wound healing. In vitro scratch assay, immunofluorescence staining and antibacterial activity of the scaffold were all studied. In vivo NAg-CCS was applied in full-thickness skin defects in Sprague-Dawley (SD) rats and the therapeutic effects of treatment were evaluated. The results showed that NAg at a concentration of 10 ppm accelerated the migration of fibroblasts with an increase in expression of α-smooth muscle actin (α-SMA). Furthermore, in vivo studies showed increased levels of pro-inflammatory and scar-related factors as well as α-SMA, while markers for macrophage activation were up-regulated. On day 60 post transplantation of ultra-thin skin graft, the regenerated skin by NAg-CCS had a similar structure to normal skin. In summary, we demonstrated that NAg-CCS was bactericidal, anti-inflammatory and promoted wound healing potentially by regulating fibroblast migration and macrophage activation, making it an ideal dermal substitute for wound regeneration.

中文翻译：

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载有银纳米颗粒的胶原蛋白/壳聚糖支架可通过调节成纤维细胞迁移和巨噬细胞活化来促进伤口愈合。

全层皮肤缺损的治疗提出了重大的临床挑战，包括感染风险和严重惊吓。银纳米颗粒（NAg）是一种有效的抗菌剂，为烧伤创口提供了一种有希望的治疗方法。但是，详细的机制仍然未知。因此，我们构建了金属纳米银颗粒-胶原蛋白/壳聚糖混合支架（NAg-CCS），并研究了其对伤口愈合的潜在影响。体外刮擦试验，支架的免疫荧光染色和抗菌活性都进行了研究。将体内NAg-CCS应用于Sprague-Dawley（SD）大鼠的全层皮肤缺损，并评估了其治疗效果。结果表明，浓度为10 ppm的NAg会加速成纤维细胞的迁移，并增加α-平滑肌肌动蛋白（α-SMA）的表达。此外，体内研究显示促炎和瘢痕相关因子以及α-SMA的水平增加，而巨噬细胞激活的标志物被上调。在超薄皮肤移植物移植后第60天，NAg-CCS再生的皮肤具有与正常皮肤相似的结构。总之，我们证明了NAg-CCS通过调节成纤维细胞迁移和巨噬细胞活化，具有杀菌，抗炎和促进伤口愈合的作用，使其成为伤口再生的理想皮肤替代品。而巨噬细胞激活的标志物被上调。在超薄皮肤移植物移植后第60天，NAg-CCS再生的皮肤具有与正常皮肤相似的结构。总之，我们证明了NAg-CCS通过调节成纤维细胞的迁移和巨噬细胞的活化，具有杀菌，抗炎和促进伤口愈合的作用，使其成为伤口再生的理想皮肤替代品。而巨噬细胞激活的标志物被上调。在超薄皮肤移植物移植后第60天，NAg-CCS再生的皮肤具有与正常皮肤相似的结构。总之，我们证明了NAg-CCS通过调节成纤维细胞迁移和巨噬细胞活化，具有杀菌，抗炎和促进伤口愈合的作用，使其成为伤口再生的理想皮肤替代品。