Enzyme Activation with a Synthetic Catalytic Co-enzyme Intermediate: Nucleotide Methylation by Flavoenzymes,Angewandte Chemie International Edition

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Enzyme Activation with a Synthetic Catalytic Co-enzyme Intermediate: Nucleotide Methylation by Flavoenzymes
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2017-09-05 06:30:44 , DOI: 10.1002/anie.201706219
Charles Bou-Nader ₁ , David Cornu ₂ , Vincent Guerineau ₃ , Thibault Fogeron ₁ , Marc Fontecave ₁ , Djemel Hamdane ₁

Affiliation

Artificial enzyme engineering is used to uncover cofactor-dependent complex mechanistic issues. A chemically synthesized N5-flavin iminium catalytic intermediate can be used to artificially activate the apoprotein version of ThyX and TrmFO for dUMP and tRNA C5 uracil methylation. This procedure bypasses the natural biosynthetic pathway and establishes this flavin iminium species as the genuine nucleotide methylating agent.

中文翻译：

合成催化辅酶中间体的酶活化：黄酮酶对核苷酸的甲基化作用

人工酶工程被用于揭示辅因子依赖性的复杂机制问题。化学合成的N5-黄素亚胺鎓催化中间体可用于人工激活ThyX和TrmFO的载脂蛋白版本，以实现dUMP和tRNA C5尿嘧啶甲基化。该程序绕过了自然的生物合成途径，并将这种黄素亚胺类物质确立为真正的核苷酸甲基化剂。

更新日期：2017-09-05

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