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Enhanced Production of κ-Carrageenase and κ-Carrageenan Oligosaccharides through Immobilization of Thalassospira sp. Fjfst-332 with Magnetic Fe3O4-Chitosan Microspheres
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-09-01 00:00:00 , DOI: 10.1021/acs.jafc.7b02869 Juanjuan Guo 1 , Zhichang Zheng 1 , Chi Chen 2 , Xu Lu 1 , Yi Zhang 1 , Baodong Zheng 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-09-01 00:00:00 , DOI: 10.1021/acs.jafc.7b02869 Juanjuan Guo 1 , Zhichang Zheng 1 , Chi Chen 2 , Xu Lu 1 , Yi Zhang 1 , Baodong Zheng 1
Affiliation
In this study, immobilized bacteria (IMB) microsphere was prepared by embedding κ-carrageenase-producing Thalassospira sp. Fjfst-332 (TF332) onto a magnetic Fe3O4-chitosan carrier. The performance of Fe3O4-chitosan carrier was optimized by comparing its bacteria immobilization capacity at different Fe3O4:chitosan ratios and temperatures, while the functions of IMB microspheres were characterized by examining their κ-carrageenase production at different temperatures, pH’s, and reuse cycles. At the 1:1 (w:w) Fe3O4:chitosan ratio, the Fe3O4-chitosan carriers possessed sufficient anchoring capacity for bacterial immobilization without significant compromise of their magnetism for magnetic separation of IMB from culture media. The spectroscopic analysis of IMB microspheres indicated that the immobilization of TF332 might affect the amide groups in chitosan. Compared to free bacteria, IMB can produce κ-carrageenase at higher temperature, wider pH range, and faster rate. More importantly, the κ-carrageenase-producing activity was sustained for at least seven reuse cycles. The major κ-carrageenan degradation products of IMB-derived κ-carrageenase were the oligosaccharides containing two to six monosaccharide units. Overall, this Fe3O4-chitosan-TF-332 microsphere has the potential to become a stable and reusable platform for large-scale production of κ-carrageenan oligosaccharides.
中文翻译:
通过固定Thalassospira sp。增强κ角叉菜胶和κ角叉菜胶寡糖的生产。具有磁性Fe 3 O 4-壳聚糖微球的Fjfst-332
在这项研究中,通过嵌入产生κ角叉菜胶的Thalassospira sp制备固定化细菌(IMB)微球。将Fjfst-332(TF332)转移到磁性Fe 3 O 4-壳聚糖载体上。通过比较其在不同Fe 3 O 4:壳聚糖比率和温度下的细菌固定能力,优化了Fe 3 O 4-壳聚糖载体的性能,同时通过在不同温度,pH值下检查它们的κ-角叉菜胶酶生成来表征IMB微球的功能。 ,以及重用周期。在1:1(w:w)Fe 3 O 4:壳聚糖比率下,Fe 3 O 4壳聚糖载体具有足够的锚固能力来固定细菌,而不会显着损害其磁性,从而使IMB从培养基中磁分离。对IMB微球的光谱分析表明,TF332的固定化可能影响壳聚糖中的酰胺基。与游离细菌相比,IMB可以在更高的温度,更宽的pH范围和更快的速率下产生κ角叉菜胶酶。更重要的是,产生κ-角叉菜胶的活性至少持续了七个重复使用周期。IMB衍生的κ-角叉菜胶酶的主要κ-角叉菜胶降解产物是含有2至6个单糖单元的寡糖。总的来说,这种Fe 3 O 4-壳聚糖-TF-332微球具有成为大规模生产κ-卡拉胶低聚糖的稳定和可重复使用的平台的潜力。
更新日期:2017-09-04
中文翻译:
通过固定Thalassospira sp。增强κ角叉菜胶和κ角叉菜胶寡糖的生产。具有磁性Fe 3 O 4-壳聚糖微球的Fjfst-332
在这项研究中,通过嵌入产生κ角叉菜胶的Thalassospira sp制备固定化细菌(IMB)微球。将Fjfst-332(TF332)转移到磁性Fe 3 O 4-壳聚糖载体上。通过比较其在不同Fe 3 O 4:壳聚糖比率和温度下的细菌固定能力,优化了Fe 3 O 4-壳聚糖载体的性能,同时通过在不同温度,pH值下检查它们的κ-角叉菜胶酶生成来表征IMB微球的功能。 ,以及重用周期。在1:1(w:w)Fe 3 O 4:壳聚糖比率下,Fe 3 O 4壳聚糖载体具有足够的锚固能力来固定细菌,而不会显着损害其磁性,从而使IMB从培养基中磁分离。对IMB微球的光谱分析表明,TF332的固定化可能影响壳聚糖中的酰胺基。与游离细菌相比,IMB可以在更高的温度,更宽的pH范围和更快的速率下产生κ角叉菜胶酶。更重要的是,产生κ-角叉菜胶的活性至少持续了七个重复使用周期。IMB衍生的κ-角叉菜胶酶的主要κ-角叉菜胶降解产物是含有2至6个单糖单元的寡糖。总的来说,这种Fe 3 O 4-壳聚糖-TF-332微球具有成为大规模生产κ-卡拉胶低聚糖的稳定和可重复使用的平台的潜力。