The present work represents the ultrasound assisted synthesis of citronellol laurate using Novozym 435 as a biocatalyst in a solvent free condition. The reaction was evaluated by two methods, one factor at a time and central composite design (CCD) by RSM. Initially, the first method produced maximum conversion up to 93.36% using optimal conditions (1:4 acid to alcohol ratio, 2% enzyme loading, 60 °C temperature, 300 rpm, 80 W power, 50% duty cycle and 25 kHz frequency). After estimation of parameters, the experimental design expert was used to evaluate the effect of molar ratio, enzyme loading, duty cycle and ultrasound power on the citronellol laurate synthesis. The optimal conditions for the ultrasound assisted synthesis of citronellol laurate was found to be: molar ratio of 1:4.2 lauric acid to citronellol, enzyme loading 2.11, ultrasound power 83.85 W and 48.54% duty cycle. Under these conditions, it was possible to obtain 96.43% of conversion in a shorter time (40 min). The Novozym 435 could be recycled in 5 successive reaction cycles. The Ordered Bi Bi kinetic model has well fitted for ultrasound assisted synthesis of citronellol laurate.