鱼粉的应用诱导海水养殖沉积物中抗生素抗性基因的繁殖,Environmental Science & Technology

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鱼粉的应用诱导海水养殖沉积物中抗生素抗性基因的繁殖
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-08-30 00:00:00 , DOI: 10.1021/acs.est.7b02875
Ying Han ₁ , Jing Wang ₁ , Zelong Zhao ₁ , Jingwen Chen ₁ , Hong Lu ₁ , Guangfei Liu ₁

Affiliation

抗生素抗性基因（ARG）在海水养殖沉积物中普遍存在，在抗生素使用的背景下，它们的存在是一个令人关注的问题。尽管大量鱼粉已释放到沉积物中，但鱼粉在ARG传播中的作用仍不清楚。在这项研究中，研究了代表性鱼粉中的高通量ARG谱图以及鱼粉对沉积物抗药性的影响。在五种鱼粉产品中总共检测到132种独特的ARG和4种移动遗传元件（MGE）。鱼粉的添加显着增加了海水养殖微观沉积物中的ARG丰度和多样性，并且ARG模式的趋势与沉积物中的常驻细菌群落有关（P<0.05）。DNase处理鱼粉去除了总ARGs的84.3％之后，鱼粉中剩余的养分增加了相对丰度，但没有增加微观沉积物中ARGs的多样性。我们的研究首次揭示了鱼粉本身是ARGs的主要储库，而鱼粉中养分引起的细菌群落的转变是塑造海水养殖微观世界沉积物中抵抗力的主要驱动力。我们的发现警告了鱼粉接收生态系统中ARG传播以前未曾意识到的风险。

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Fishmeal Application Induces Antibiotic Resistance Gene Propagation in Mariculture Sediment

Antibiotic resistance genes (ARGs) are globally prevalent in mariculture sediment, and their presence is an issue of concern in the context of antibiotic use. Although large amounts of fishmeal have been released into the sediment, the role of fishmeal in ARG dissemination remains unclear. In this study, high-throughput ARG profiles in representative fishmeal products and the impact of fishmeal on the sediment resistome were investigated. A total of 132 unique ARGs and 4 mobile genetic elements (MGEs) were detected in five fishmeal products. ARG abundance and diversity in the mariculture microcosm sediment were significantly increased by the addition of fishmeal, and trends in ARG patterns correlated with the resident bacterial community in sediment (P < 0.05). After DNase treatment of fishmeal removed 84.3% of total ARGs, the remaining nutrients in fishmeal increased the relative abundance but not the diversity of ARGs in microcosm sediment. Our study has revealed for the first time that fishmeal itself is a major reservoir for ARGs, and the shift in the bacterial community induced by the nutrients in fishmeal is the main driver shaping the resistome in mariculture microcosm sediment. Our findings caution against the previously unperceived risk of ARG propagation in fishmeal-receiving ecosystems.

更新日期：2017-08-30

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