集成微电渗析的微型液体电极放电-光发射光谱系统，用于血清钾筛选<a href="#fn1" title="提供电子补充信息（ESI）。参见DOI：10.1039 / c7ja00111h">†</a>,Journal of Analytical Atomic Spectrometry

当前位置： X-MOL 学术 › J. Anal. At. Spectrom. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

集成微电渗析的微型液体电极放电-光发射光谱系统，用于血清钾筛选 †
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2017-05-05 00:00:00 , DOI: 10.1039/c7ja00111h
Shuang Liu _{1,

2,

3,

4,

5} , Yi Cai _{1,

2,

3,

4,

5} , Yong-Liang Yu _{1,

2,

3,

4,

5} , Jian-Hua Wang _{1,

2,

3,

4,

5}

Affiliation

自动化微电渗析（μED）-液体电极放电（LED）-光发射光谱（OES）系统的开发旨在对血清中的钾进行筛查，特别是对于微量样品。钾在血清是上线提取经由一个μED过程，并随后引入到LED微等离子体在执行光发射检测λ _EM766.5 nm。使用电荷耦合器件（CCD）光谱仪测量钾的光发射强度，以进行定量分析。研究了一些重要的参数，这些参数决定了用μED萃取钾和用LED-OES检测钾的能力。本系统通过每次分析消耗20μL的血清样品量，具有消除基质干扰的出色能力，蛋白质去除效率> 99％。整个分析过程，包括样品引入，预处理和检测，不超过90 s。检出限为0.6 mg L ^-1，线性范围为2–70 mg L ^-1，在5 mg L ^-1时的RSD值为4.8％钾。μED-LED-OES系统的实际适用性通过测定血清认证参考物质和真实人类血清样品中的钾来证明。本系统提供了用于筛选患者中钾浓度变化的潜在策略。

"点击查看英文标题和摘要"

A miniature liquid electrode discharge-optical emission spectrometric system integrating microelectrodialysis for potassium screening in serum†

An automated microelectrodialysis (μED)-liquid electrode discharge (LED)-optical emission spectrometric (OES) system was developed with the aim of potassium screening in serum, especially for a micro-sample volume. Potassium in serum was on-line extracted via a μED process and subsequently introduced into the LED microplasma to perform the optical emission detection at λ_em 766.5 nm. The optical emission intensity of potassium was measured using a charge-coupled device (CCD) spectrometer for quantitative analysis. A few important parameters governing the extraction of potassium by μED and its detection by LED-OES were investigated. The present system provides an excellent capability for eliminating matrix interference with a protein removal efficiency of >99%, by consuming a serum sample volume of 20 μL for each analysis. The entire analytical process, including sample introduction, pretreatment and detection, takes no more than 90 s. A detection limit of 0.6 mg L⁻¹ was obtained along with a linear range of 2–70 mg L⁻¹ and a RSD value of 4.8% at 5 mg L⁻¹ potassium. The practical applicability of the μED-LED-OES system was demonstrated by the determination of potassium in serum certified reference materials and real human serum samples. The present system offers a potential strategy for screening the variation of the concentration of potassium in patients.

更新日期：2017-05-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南