Inappropriate use of ethanol (EtOH) had led to noticeable health problems, but a beneficial phenomenon was found that EtOH displayed unique influences for toxic trans-crotonaldehyde (TCA) derived from mitochondrial lipid peroxidation. The influences of EtOH on the structure of TCA were systematically probed by UV-vis & Raman spectroscopy in the absence and presence of mitochondria, respectively. The maximum UV-vis peak at 301 nm of TCA was red shifted by hydroxyl (-OH) and methyl (-CH₃) of EtOH, respectively. Raman stretching band of aldehyde (-CH=O) of TCA (TCA-CH=O) was split by the -CH₃ of EtOH. The -CH₃ increased TCA-CH=O stretching frequency while the -OH induced it. The more exposed -OH, the less stretching frequency. The ectopic -CH₃ red shifted the UV-vis peak at 301 nm and Raman band of TCA-CH=O. In mitochondria, EtOH red shifted Raman stretching band of TCA-CH=O. Raman stretching bands of C-H, C-O and C-C of EtOH were red shifted, while Raman stretching bands of -CH₂ and C-C-O of EtOH disappeared. The paper unearths the influences of EtOH to trap and transform the structure of TCA-CH=O. This discovery has an important contribution to eliminate TCA in order to protect and repair mtDNA by means of the decrease of 8-oxoG.

中文翻译：

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乙醇对大鼠心肌线粒体中有毒反式巴豆醛结构的影响。

乙醇（EtOH）的不当使用导致了明显的健康问题，但是发现了一个有益的现象，即乙醇对线粒体脂质过氧化衍生的有毒反式巴豆醛（TCA）表现出独特的影响。在不存在和存在线粒体的情况下，分别通过UV-vis和拉曼光谱系统地探讨了EtOH对TCA结构的影响。TCA在301 nm处的最大UV-vis峰分别被EtOH的羟基（-OH）和甲基（-CH ₃）红移。TCA的醛（-CH ＝ O）的拉曼拉伸带（TCA-CH ＝ O）被EtOH的-CH ₃分开。-CH ₃增加了TCA-CH = O的拉伸频率，而-OH诱导了它。-OH暴露的越多，拉伸频率越小。异位-CH ₃红色移动了301 nm处的UV-vis峰，TCA-CH = O的拉曼光谱带。在线粒体中，EtOH红移了TCA-CH = O的拉曼拉伸带。EtOH的CH，CO和CC的拉曼拉伸带发生红移，而EtOH的-CH ₂和CCO的拉曼拉伸带消失。本文发掘了EtOH的影响，以捕获和转化TCA-CH = O的结构。这一发现对消除TCA具有重要贡献，从而通过减少8-oxoG来保护和修复mtDNA。