酰基辅酶 A：单酰基甘油酰基转移酶 3 (MGAT3) 基因是小鼠的假基因，但在大鼠中编码功能性酶。,Lipids

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酰基辅酶 A：单酰基甘油酰基转移酶 3 (MGAT3) 基因是小鼠的假基因，但在大鼠中编码功能性酶。
Lipids ( IF 1.8 ) Pub Date : 2011 Jun , DOI: 10.1007/s11745-011-3537-1
Yong Gang Yue ₁ , Yan Qun Chen , Youyan Zhang , He Wang , Yue-Wei Qian , Jeffrey S Arnold , John N Calley , Shuyu D Li , William L Perry , Hong Y Zhang , Robert J Konrad , Guoqing Cao

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甘油三酯 (TAG) 吸收涉及其初始水解为脂肪酸和单酰基甘油 (MAG)，然后在肠细胞内重新合成为二酰基甘油 (DAG) 和 TAG。DAG 的再合成由依赖于脂肪酰基辅酶 A 的单酰基甘油酰基转移酶 (MGAT) 促进。已在人类中分离出三种 MGAT 酶，MGAT2 和 MGAT3 在肠道中的表达表明它们在 TAG 吸收中的功能作用。在本文中，我们报告 Mogat3 基因在小鼠中似乎是一个假基因，而在大鼠中它是一个功能基因。对小鼠基因组 Mogat3 序列的检查揭示了会导致翻译终止密码子或移码的多重变化。然而，预测大鼠 Mogat3 基因编码 362 个氨基酸的功能性酶。大鼠 MGAT3 在人胚胎肾 293 (HEK293) 细胞中的表达导致形成 36 kDa 蛋白，该蛋白显示显着的 MGAT 但不显示 DGAT 活性。通过实时PCR分析对大鼠MGAT3的组织表达分析表明大鼠MGAT3在肠和胰腺中具有高水平表达。因此，我们的结果为理解 MGAT2 和 MGAT3 的相对功能作用以及未来在动物模型中探索 MGAT3 功能提供了分子基础。

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The acyl coenzymeA:monoacylglycerol acyltransferase 3 (MGAT3) gene is a pseudogene in mice but encodes a functional enzyme in rats.

Triglyceride (TAG) absorption involves its initial hydrolysis to fatty acids and monoacylglycerol (MAG), which are resynthesized back to diacylglycerol (DAG) and TAG within enterocytes. The resynthesis of DAG is facilitated by fatty acyl-CoA dependent monoacylglycerol acyltransferases (MGATs). Three MGAT enzymes have been isolated in humans and the expression of MGAT2 and MGAT3 in the intestines suggests their functional role in the TAG absorption. In this paper, we report that the Mogat3 gene appears to be a pseudogene in mice while it is a functional gene in rats. Examination of the mouse genomic Mogat3 sequence revealed multiple changes that would result in a translational stop codon or frameshifts. The rat Mogat3 gene, however, is predicted to encode a functional enzyme of 362 amino acids. Expression of rat MGAT3 in human embryonic kidney 293 (HEK293) cells led to the formation of a 36-kDa protein that displayed significant MGAT but not DGAT activity. Tissue expression analysis of rat MGAT3 by real-time PCR analysis indicated that rat MGAT3 has a high level of expression in intestines and pancreas. Our results thus provide the molecular basis to understand the relative functional role of MGAT2 and MGAT3 and also for future exploration of MGAT3 function in animal models.

更新日期：2017-01-31

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