Guanosine monophosphate, among the nucleotides, has the unique property to self-associate and form nanoscale cylinders consisting of hydrogen-bonded G-quartet disks, which are stacked on top of one another. Such self-assemblies describe not only the basic structural motif of G-quadruplexes formed by, e.g., telomeric DNA sequences, but are also interesting targets for supramolecular chemistry and nanotechnology. The G-quartet stacks serve as an excellent model to understand the fundamentals of their molecular self-association and to unveil their application spectrum. However, the thermodynamic stability of such self-assemblies over an extended temperature and pressure range is largely unexplored. Here, we report a combined FTIR and NMR study on the temperature and pressure stability of G-quartet stacks formed by disodium guanosine 5'-monophosphate (Na₂5'-GMP). We found that under abyssal conditions, where temperatures as low as 5 °C and pressures up to 1 kbar are reached, the self-association of Na₂5'-GMP is most favoured. Beyond those conditions, the G-quartet stacks dissociate laterally into monomer stacks without significantly changing the longitudinal dimension. Among the tested alkali cations, K⁺ is the most efficient one to elevate the temperature as well as the pressure limits of GMP self-assembly.

中文翻译：

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鸟苷一磷酸鸟苷自组装体的温度和压力极限。

核苷酸中的鸟嘌呤单磷酸具有独特的性质，可以自我缔合并形成由氢键合的G四方圆盘组成的纳米级圆柱，它们相互堆叠。这样的自组装不仅描述了由例如端粒DNA序列形成的G-四链体的基本结构基序，而且还是超分子化学和纳米技术的有趣靶标。G四重奏堆栈是了解分子自我缔合的基本原理并揭示其应用范围的绝佳模型。但是，这种自组装在扩展的温度和压力范围内的热力学稳定性在很大程度上尚未得到开发。在这里，我们报告了结合的FTIR和NMR研究由鸟苷二钠5'形成的G四重奏堆栈的温度和压力稳定性₂ 5'-GMP）。我们发现在深海条件下，温度低至5°C，压力高达1 kbar，Na ₂ 5'-GMP的自缔合是最有利的。在这些条件之外，G四重奏堆栈在不显着改变纵向尺寸的情况下从侧面分解为单体堆栈。在测试的碱金属阳离子中，K ⁺是提高温度和GMP自组装压力极限的最有效的阳离子。