Development of a Silicon-Rhodamine Based Near-Infrared Emissive Two-Photon Fluorescent Probe for Nitric Oxide,Analytical Chemistry

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Development of a Silicon-Rhodamine Based Near-Infrared Emissive Two-Photon Fluorescent Probe for Nitric Oxide
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-08-29 00:00:00 , DOI: 10.1021/acs.analchem.7b02697
Zhiqiang Mao ₁ , Hong Jiang ₁ , Xinjian Song ₁ , Wei Hu ₁ , Zhihong Liu ₁

Affiliation

Two-photon (TP) fluorescent probes are potential candidates for near-infrared (NIR) imaging which holds great promise in biological research. However, currently, most TP probes emit at wavelength <600 nm, which impedes their practical applications. In this work, we explored the TP properties of a silicon-rhodamine (SiR) derivative and hence developed the first SiR scaffold based “NIR-to-NIR” TP probe (SiRNO) for nitric oxide (NO). SiRNO exhibited high sensitivity and specificity, as well as fast response for NO detection. It was able to track the subtle variation of intracellular NO content in live cells. Owing to the NIR excitation and emission, SiRNO enabled the detection of NO in situ in the xenograft tumor mouse model, revealing the NO generation during the tumor progression. This work indicates that SiR can be an ideal platform for the development of NIR emissive TP probe and may thus promote the advancement of NIR imaging.

中文翻译：

一氧化氮的基于若丹明的近红外发射双光子荧光探针的开发

两光子（TP）荧光探针是近红外（NIR）成像的潜在候选者，在生物学研究中具有广阔的前景。但是，目前，大多数TP探针发射的波长<600 nm，这阻碍了它们的实际应用。在这项工作中，我们探索了硅罗丹明（SiR）衍生物的TP特性，因此开发了第一个基于SiR支架的一氧化氮（NO）的“ NIR-to-NIR” TP探针（SiRNO）。SiRNO表现出高灵敏度和特异性，以及对NO检测的快速响应。它能够追踪活细胞中细胞内NO含量的细微变化。由于近红外激发和发射，SiRNO能够在异种移植肿瘤小鼠模型中原位检测NO，从而揭示了肿瘤进展过程中的NO生成。这项工作表明，SiR可以成为开发近红外发射TP探针的理想平台，从而可以促进近红外成像的发展。

更新日期：2017-08-29

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