The 1,3-alternate 25,27-bis-[p-chlorobenzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene-bonded silica gel stationary phase was synthesized, structurally characterized, and used as a selector in high performance liquid chromatography. Selectivity studies on that phase used aromatic positional isomers, alkylbenzenes, polynuclear aromatic hydrocarbons, sulfonamides, and non-steroidal anti-inflammatory drugs as analytes. The effects of organic modifier content and pH of the mobile phase on retention and selectivity of selected aromatic positional isomers were studied. Selectivity comparisons of the novel phase vs. 1,3-alternate 25,27-di-[benzyloxy]-26,28-bis-[3-propyloxy]-calix[4]arene phase and commercially available RP-Phenyl phases were performed. The retention mechanism was also discussed. The results indicated that the calixarene stationary phase behaves like a reversed-phase packing; however, other retention mechanisms seem to be involved in the separation process.

中文翻译：

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新型1,3,25,27-双-[对氯苄氧基] -26,28-双-[3-丙氧基]-杯[4]芳烃硅胶键合固定相的制备和HPLC评价。

合成1,3,25,27-双-[对-氯苄氧基] -26,28-双-[3-丙氧基]-杯[4]芳烃键合硅胶固定相，结构表征，并用作高效液相色谱中的选择器。在该相的选择性研究中，使用了芳族位置异构体，烷基苯，多核芳烃，磺酰胺和非甾体抗炎药作为分析物。研究了有机改性剂含量和流动相的pH对所选芳族位置异构体的保留度和选择性的影响。进行了新型相与1,3-交替25,27-二-[苄氧基] -26,28-双-[3-丙氧基]-杯[4]芳烃相和市售RP-苯基相的选择性比较。还讨论了保留机制。结果表明，杯芳烃固定相表现为反相堆积。但是，其他保留机制似乎与分离过程有关。