A new hydrophilic interaction liquid chromatography method for the simultaneous determination of ascorbic acid (AA), erythorbic acid (EA), 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) and 2-O-beta-D-glucopyranosyl-L-ascorbic acid (AA-2betaG) was developed using a diol column with an isocratic solution of acetonitrile-66.7 mM ammonium acetate solution (85:15, v/v) at a detection wavelength of 260 nm. The calibration curves were found to be linear in the range of 1-50 microg/ml for AA and EA and in the range of 2.5-100 microg/ml for AA-2G and AA-2betaG. Detection limits of AA, EA, AA-2G and AA-2betaG were 0.3, 0.3, 0.03 and 0.03 microg/ml, respectively. This method was satisfactorily applied to the determination of AA, EA, AA-2G and AA-2betaG in a fruit, a food and beverages. The results show that the procedure is simple and sensitive and that it can be employed for the simultaneous determination of AA and its related compounds in foods and beverages.

中文翻译：

---

亲水作用液相色谱法测定食品和饮料中的抗坏血酸及其相关化合物。

一种同时测定抗坏血酸（AA），异抗坏血酸（EA），2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AA-2G）和2-O-β- D-吡喃葡萄糖基-L-抗坏血酸（AA-2betaG）使用二元醇色谱柱与乙腈-66.7 mM乙酸铵溶液（85:15，v / v）的等度溶液开发，检测波长为260 nm。发现校准曲线对于AA和EA在1-50微克/毫升的范围内，对于AA-2G和AA-2betaG在2.5-100微克/毫升的范围内是线性的。AA，EA，AA-2G和AA-2betaG的检出限分别为0.3、0.3、0.03和0.03微克/毫升。该方法适用于水果，食品和饮料中AA，EA，AA-2G和AA-2betaG的测定。