A simplex-centroid mixture design (SCMD) approach was used to select the best solvent for the extraction of the phenolic compounds from Scirpus holoschoenus L. rhizome. The optimized crude acetone extract (CE) and its ethyl acetate (EA) and petroleum ether (PE) fractions were investigated for their antioxidant and antibacterial properties. The EA fraction showed the highest antioxidant activity and antibacterial effect, with minimal inhibitory concentration (MIC) values of 0.4 and 0.6 mg mL⁻¹ for Staphylococcus aureus and Bacillus subtilis, respectively. The antibacterial activity was evaluated by SCMD and the results indicated that antagonist binary extract effects between the PE–EA and PE–CE pairs are found.

使用单纯形-质心混合物设计（SCMD）方法选择最佳溶剂，以从拟南芥中提取酚类化合物。研究了优化的丙酮粗提物（CE）及其乙酸乙酯（EA）和石油醚（PE）馏分的抗氧化和抗菌性能。EA级分显示出最高的抗氧化活性和抗菌效果，对金黄色葡萄球菌和枯草芽孢杆菌的最小抑菌浓度（MIC）分别为0.4和0.6 mg mL ^-1。通过SCMD评估了抗菌活性，结果表明在PE–EA和PE–CE对之间发现了拮抗二元提取物作用。