As the only endogenous coreactant in the electrochemiluminescence (ECL) system, the dissolved O₂ was the ideal candidate due to the mild reaction and easy operation, but compared to S₂O₈^2–, the dissolved O₂ with weaker redox activity suffers from the poor enhancement effect of the luminophore, which restricted the further application in bioanalysis. Here, a high-intense ECL signal was gained by the employing of Pt nanomaterials as a coreaction accelerator to generate more of the intermediate of dissolved O₂ to promote the coreaction efficiency. On the basis of a new ternary ECL system of Pt nanomaterials as the coreaction accelerator, dissolved O₂ as the coreactant, and a neotype rubrene microrods as the luminophore, an efficient “on–off–on” solid-state ECL switch platfrom was designed for ultrasensitive microRNA (miRNA) detection with a background reduction strategy of ferrocene-labeled single-stranded DNA (Fc-DNA) as a quencher. In the presence of miRNA 141, the Pt nanoparticles labeled hairpin (HP1/PtNPs) was opened to produce plenty of Pt nanoparticles labeled output DNA (S1/PtNPs) and release the miRNA-141 to participate in the next cycle. Then, the S1/PtNPs were captured on the surface of the electrode by the complementary strand to obtain the super “signal on” state with extremely high ECL signal. This novel solid-state ECL platform exhibited excellent sensitivity from 10 aM to 100 pM with a detection limit of 2.1 aM, which provided a new approach for ultrasensitive ECL bioanalysis.

中文翻译：

---

基于钌微棒作为发光体和Pt纳米材料作为共作用促进剂的三级电化学发光系统，用于从癌细胞中超敏感地检测MicroRNA。

作为电致发光（ECL）系统中唯一的内源性共反应物，溶解的O ₂由于反应温和且易于操作而成为理想的候选物，但与S ₂ O ₈ ^2–相比，氧化还原活性较弱的溶解的O ₂具有以下缺点：发光体的增强效果差，限制了其在生物分析中的进一步应用。在这里，通过使用Pt纳米材料作为共作用促进剂来产生高强度的ECL信号，以产生更多的溶解的O ₂中间体，从而提高了共作用效率。在以Pt纳米材料为共作用促进剂的新型三元ECL体系的基础上，溶解了O ₂作为一种共激活剂，一种新型的红荧烯微棒作为发光体，设计了一种高效的“ on-off-on”固态ECL开关平台，用于超敏感的microRNA（miRNA）检测，并采用二茂铁标记的单链DNA的背景还原策略（Fc-DNA）作为淬灭剂。在存在miRNA 141的情况下，将Pt纳米颗粒标记的发夹（HP1 / PtNPs）打开，以产生大量的Pt纳米颗粒标记的输出DNA（S1 / PtNPs），并释放miRNA-141以参与下一个周期。然后，S1 / PtNPs被互补链捕获在电极表面，从而获得具有极高ECL信号的超级“信号开启”状态。这个新型的固态ECL平台在10 aM至100 pM的灵敏度范围内具有2.1 aM的检测极限，为超灵敏ECL生物分析提供了一种新方法。