支持的脂质双层上的平末端DNA平铺的远距离排序。,Journal of the American Chemical Society

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支持的脂质双层上的平末端DNA平铺的远距离排序。
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-08-21 00:00:00 , DOI: 10.1021/jacs.7b06572
Nicole Avakyan ₁ , Justin W. Conway ₁ , Hanadi F. Sleiman ₁

Affiliation

使用原子力显微镜研究了脂质双层上末端钝的DNA交叉片段的远距离排序。“平端”磁贴没有单链互补端，因此，可以通过π堆叠其双端进行交互，而不是通过碱基配对进行组装。这项工作表明，DNA双链体末端之间的基本π堆积相互作用，胆固醇介导的DNA锚定和静电DNA与支持的脂质双层（SLB）的结合之间的平衡提供了构建长距离有序动态材料的机会。软支持。所显示的瓷砖在微米尺度上组织成新颖的可调表面堆积形态。这项工作着眼于未修饰或用胆固醇单位修饰的三点星形（3PS）瓷砖，并研究了它们在支持的脂质双层上的相互作用。在流体双层上，胆固醇块形成几乎没有缺陷的扩展的六边形阵列，而未修饰的块不结合。相反，修饰和未修饰的瓷砖都结合到凝胶相双层上，并产生新的有组织形态的阵列。随着瓷砖浓度的增加，我们观察到一系列图案，这些图案逐渐倾向于瓷砖-瓷砖堆积而不是双端π-堆积。这些结构可以选择性地图案化相分离的脂质双层的结构域，并且在四臂交叉砖中也观察到了图案。动态钝端触点可促进纠错和网络重新配置，以最大程度地提高与基板的良好相互作用，这对于观察到的瓷砖组织而言是必需的。这些结果表明，小的平头瓷砖可以用作平台，将寡核苷酸，纳米颗粒和蛋白质组织成与生物学相关的膜系统或其他软表面材料相接的广泛网络，以用于细胞识别，等离激元，光收集，膜蛋白装配或分析设备的模型系统。

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Long-Range Ordering of Blunt-Ended DNA Tiles on Supported Lipid Bilayers

Long-range ordering of DNA crossover tiles with blunt ends on lipid bilayers is investigated using atomic force microscopy. “Blunt-ended” tiles do not have single-stranded complementary ends, and thus instead of assembling via base-pairing, they can interact by π-stacking of their duplex ends. This work demonstrates that the balance of base π-stacking interactions between the ends of DNA duplexes, cholesterol-mediated DNA anchoring, and electrostatic DNA binding to supported lipid bilayers (SLBs) presents an opportunity to build dynamic materials with long-range order on a soft support. The tiles are shown to organize into novel tunable surface packing morphologies on the micrometer scale. This work focuses on three-point star (3PS) tiles that are either unmodified or modified with a cholesterol unit and investigates their interactions on supported lipid bilayers. On fluid bilayers, the cholesterol tiles form extended hexagonal arrays with few defects, while the unmodified tiles do not bind. In contrast, both modified and unmodified tiles bind to gel-phase bilayers and produce arrays of new organized morphologies. With increasing tile concentration, we observe a range of motifs, that progressively favor tile–tile packing over duplex-end π-stacking. These structures can selectively pattern domains of phase-separated lipid bilayers, and the patterning is also observed for four-arm cross-tiles. Dynamic blunt end contacts promote error correction and network reconfiguration to maximize favorable interactions with the substrate and are required for the observed tile organization. These results suggest that small blunt-ended tiles can be used as a platform to organize oligonucleotides, nanoparticles, and proteins into extensive networks at the interface with biologically relevant membrane systems or other soft surface materials for applications in cellular recognition, plasmonics, light harvesting, model systems for membrane protein assemblies, or analytical devices.

更新日期：2017-08-21

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