粘弹性流体和牛顿流体同流的片上微粒和细胞洗涤,Analytical Chemistry

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粘弹性流体和牛顿流体同流的片上微粒和细胞洗涤
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-08-17 00:00:00 , DOI: 10.1021/acs.analchem.7b02671
Dan Yuan ₁ , Say Hwa Tan ₂ , Ronald Sluyter _{3,

4} , Qianbin Zhao ₁ , Sheng Yan ₁ , N. T. Nguyen ₂ , Jinhong Guo ₅ , Jun Zhang ₆ , Weihua Li ₁

Affiliation

这项工作研究了在直接的微通道中使用粘弹性流体和牛顿流体的同流配置对微粒和细胞进行芯片上清洗的过程。通过将少量生物相容性聚合物添加到颗粒培养基或细胞培养基中，诱导的粘弹性可以将颗粒和细胞从其原始培养基横向推向同流牛顿培养基。此行为可用于粒子或细胞清洗。首先，我们展示了使用粘弹性流体和牛顿流体的并流，通过尺寸依赖的迁移速度进行的片上颗粒洗涤。确定有效洗涤颗粒的临界粒度。其次，我们证明了使用粘弹性流体和牛顿流体的并流对Jurkat细胞进行连续的芯片清洗。研究了Jurkat细胞沿通道长度的横向迁移过程。另外，通过血细胞计数和流式细胞术验证了细胞洗涤质量，回收率高达92.8％。来自两个入口和两个出口的培养基的扫描分光光度测量表明，同流的扩散可忽略不计，表明从培养基到磷酸盐缓冲盐水培养基的有效细胞洗涤。这项技术可能是乏味的常规离心方法的一种更安全，更简单，更便宜，更有效的替代方法，并且可以开拓广泛的生物医学应用领域。来自两个入口和两个出口的培养基的扫描分光光度测量表明，同流的扩散可忽略不计，表明从培养基到磷酸盐缓冲盐水培养基的有效细胞洗涤。这项技术可能是乏味的常规离心方法的一种更安全，更简单，更便宜，更有效的替代方法，并且可以开拓广泛的生物医学应用领域。来自两个入口和两个出口的培养基的扫描分光光度测量表明，同流的扩散可忽略不计，表明从培养基到磷酸盐缓冲盐水培养基的有效细胞洗涤。这项技术可能是乏味的常规离心方法的一种更安全，更简单，更便宜，更有效的替代方法，并且可以开拓广泛的生物医学应用领域。

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On-Chip Microparticle and Cell Washing Using Coflow of Viscoelastic Fluid and Newtonian Fluid

This work investigates the on-chip washing process of microparticles and cells using coflow configuration of viscoelastic fluid and Newtonian fluid in a straight microchannel. By adding a small amount of biocompatible polymers into the particle medium or cell culture medium, the induced viscoelasticity can push particles and cells laterally from their original medium to the coflow Newtonian medium. This behavior can be used for particle or cell washing. First, we demonstrated on-chip particle washing by the size-dependent migration speed using coflow of viscoelastic fluid and Newtonian fluid. The critical particle size for efficient particle washing was determined. Second, we demonstrated continuous on-chip washing of Jurkat cells using coflow of viscoelastic fluid and Newtonian fluid. The lateral migration process of Jurkat cells along the channel length was investigated. In addition, the cell washing quality was verified by hemocytometry and flow cytometry with a recovery rate as high as 92.8%. Scanning spectrophotometric measurements of the media from the two inlets and the two outlets demonstrated that diffusion of the coflow was negligible, indicating efficient cell washing from culture medium to phosphate-buffered saline medium. This technique may be a safer, simpler, cheaper, and more efficient alternative to the tedious conventional centrifugation methods and may open up a wide range of biomedical applications.

更新日期：2017-08-17

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