Multilayer Choline Phosphate Molecule Modified Surface with Enhanced Cell Adhesion but Resistance to Protein Adsorption,Langmuir

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Multilayer Choline Phosphate Molecule Modified Surface with Enhanced Cell Adhesion but Resistance to Protein Adsorption
Langmuir ( IF 3.7 ) Pub Date : 2017-08-11 00:00:00 , DOI: 10.1021/acs.langmuir.7b01050
Xingyu Chen _{1,

2} , Ming Yang ₁ , Botao Liu ₁ , Zhiqiang Li ₃ , Hong Tan ₁ , Jianshu Li ₁

Affiliation

Choline phosphate (CP), which is a new zwitterionic molecule, and has the reverse order of phosphate choline (PC) and could bind to the cell membrane though the unique CP–PC interaction. Here we modified a glass surface with multilayer CP molecules using surface-initiated atom-transfer radical polymerization (SI-ATRP) and the ring-opening method. Polymeric brushes of (dimethylamino)ethyl methacrylate (DMAEMA) were synthesized by SI-ATRP from the glass surface. Then the grafted PDMAEMA brushes were used to introduce CP groups to fabricate the multilayer CP molecule modified surface. The protein adsorption experiment and cell culture test were used to evaluate the biocompatibility of the modified surfaces by using human umbilical veinendothelial cells (HUVECs). The protein adsorption results demonstrated that the multilayer CP molecule decorated surface could prevent the adsorption of fibrinogen and serum protein. The adhesion and proliferation of cells were improved significantly on the multilayer CP molecule modified surface. Therefore, the biocompatibility of the material surface could be improved by the modified multilayer CP molecule, which exhibits great potential for biomedical applications, e.g., scaffolds in tissue engineering.

中文翻译：

多层胆碱磷酸分子修饰的表面，具有增强的细胞粘附力，但对蛋白质吸附具有抵抗力

磷酸胆碱（CP）是一种新的两性离子分子，具有磷酸胆碱（PC）的相反顺序，并且可以通过独特的CP-PC相互作用与细胞膜结合。在这里，我们使用表面引发的原子转移自由基聚合（SI-ATRP）和开环方法对具有多层CP分子的玻璃表面进行了改性。通过SI-ATRP从玻璃表面合成了甲基丙烯酸（二甲基氨基）乙酯（DMAEMA）的聚合物刷。然后，将接枝的PDMAEMA刷用于引入CP基团以制造多层CP分子改性的表面。通过人脐静脉内皮细胞（HUVEC），利用蛋白质吸附实验和细胞培养实验评估改性表面的生物相容性。蛋白质吸附结果表明，多层CP分子修饰的表面可以阻止纤维蛋白原和血清蛋白质的吸附。在多层CP分子修饰的表面上，细胞的粘附和增殖显着改善。因此，可以通过修饰的多层CP分子改善材料表面的生物相容性，该修饰的多层CP分子对于生物医学应用（例如组织工程中的支架）具有巨大的潜力。

更新日期：2017-08-12

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