Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The engineered C. tyrobutyricum strain efficiently fermented the inulin-type carbohydrates from Jerusalem artichoke, without any pretreatment being necessary for the production of hydrogen. A comparatively high hydrogen yield (3.7 mol/mol inulin-type sugar) was achieved after 96 h in a batch process with simultaneous saccharification and fermentation (SSF), with an overall volumetric productivity rate of 620 ± 60 mL/h/L when the initial total sugar concentration of the inulin extract was increased to 100 g/L. Synthesis of inulinase in the batch SSF culture was closely associated with strain growth until the end of the exponential phase, reaching a maximum activity of 28.4 ± 0.26 U/mL. The overall results show that the highly productive and abundant biomass crop Jerusalem artichoke can be a good substrate for hydrogen production, and that the application of batch SSF for its conversion has the potential to become a cost-effective process in the near future.

中文翻译：

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表达外菊糖酶基因的酪丁酸梭菌从耶路撒冷洋蓟发酵产氢。

据报道，酪丁酸梭状芽孢杆菌ATCC25755能够产生大量的氢气。在这项研究中，从多粘芽孢杆菌SC-2克隆的外切菊粉酶编码基因进入表达质粒pSY6，并在酪丁酸梭菌细胞中表达。工程化的酪丁酸梭菌菌株有效地发酵了菊芋中的菊粉型碳水化合物，而无需进行任何预处理即可生产氢气。在分批处理过程中，同时进行糖化和发酵（SSF）的分批处理过程96小时后，获得了较高的氢气产量（3.7 mol / mol菊粉型糖），当分馏时，总体积生产率为620±60 mL / h / L。菊粉提取物的初始总糖浓度增加到100 g / L。批量SSF培养物中菊粉酶的合成与菌株生长密切相关，直到指数期结束，最大活性达到28.4±0.26 U / mL。总体结果表明，高产和丰富的生物量农作物菊芋可以作为制氢的良好基质，并且在不久的将来应用分批SSF进行转化有可能成为具有成本效益的过程。