Advancing Biocapture Substrates via Chemical Lift-Off Lithography,Chemistry of Materials

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Advancing Biocapture Substrates via Chemical Lift-Off Lithography
Chemistry of Materials ( IF 7.2 ) Pub Date : 2017-08-11 00:00:00 , DOI: 10.1021/acs.chemmater.7b01970
Huan H. Cao _{1,

2} , Nako Nakatsuka _{1,

2} , Wei-Ssu Liao _{1,

2} , Andrew C. Serino _{1,

2,

3} , Sarawut Cheunkar _{1,

2} , Hongyan Yang ₄ , Paul S. Weiss _{1,

2,

3} , Anne M. Andrews _{1,

2,

4}

Affiliation

Creating small-molecule-functionalized platforms for high-throughput screening or biosensing applications requires precise placement of probes on solid substrates and the ability to capture and to sort targets from multicomponent samples. Here, chemical lift-off lithography was used to fabricate large-area, high-fidelity patterns of small-molecule probes. Lift-off lithography enables biotin–streptavidin patterned recognition with feature sizes ranging from micrometers to below 30 nm. Subtractive patterning via lift-off facilitated insertion of a different type of molecule and, thus, multiplexed side-by-side placement of small-molecule probes such that binding partners were directed to cognate probes from solution. Small molecules mimicking endogenous neurotransmitters were patterned using lift-off lithography to capture native membrane-associated receptors. We characterized patterning of alkanethiols that self-assemble on Au having different terminal functional groups to expand the library of molecules amenable to lift-off lithography enabling a wide range of functionalization chemistries for use with this simple and versatile patterning method.

中文翻译：

通过化学剥离光刻技术发展生物捕获基质

创建用于高通量筛选或生物传感应用的小分子功能化平台，需要在固体基质上精确放置探针，并具有从多组分样品中捕获和分类靶标的能力。在这里，化学剥离光刻技术用于制造大面积，高保真度的小分子探针图案。剥离光刻技术可实现生物素-链霉亲和素图案识别，其特征尺寸范围从微米到30 nm以下。通过剥离的减法构图促进了不同类型分子的插入，并因此促进了小分子探针的并排多重放置，从而使结合伴侣从溶液中定向至同源探针。模拟模拟内源性神经递质的小分子使用剥离光刻技术来捕获天然的膜相关受体。我们表征了烷硫醇的构图，该构图在具有不同末端官能团的Au上自组装，从而扩展了可进行平版印刷术的分子库，从而使多种功能化化学试剂可用于此简单而通用的构图方法。

更新日期：2017-08-11

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