It is crucial to develop highly sensitive and selective probes toward hydrazine because it is a class of highly toxic and pollutant compound. Herein, using fracture of carbon carbon double bond and dissociation of amide by hydrazine, a novel off-on fluorescent probe was developed for hydrazine. The probe can quantitatively detect hydrazine in concentration range from 0 to 20 μM with the LOD of 140 nM. Further, it displayed excellent selectivity and anti-interference ability over many neutral molecules, metal ions, anions, and biological species. The ability to target lysosome and the response of hydrazine to this probe in a living cell was successfully tracked via fluorescence imaging.

开发针对肼的高度敏感和选择性的探针至关重要，因为它是一类剧毒和高污染的化合物。在本文中，利用碳碳双键的断裂和酰胺被肼解离，开发了用于肼的新型脱落荧光探针。该探针可以定量检测0至20μM范围内的肼，LOD为140 nM。此外，它对许多中性分子，金属离子，阴离子和生物物种显示出优异的选择性和抗干扰能力。通过荧光成像成功地追踪了靶向溶酶体的能力以及肼对活细胞中该探针的反应。