Comprehensive Proteomic Analysis of PGC7-Interacting Proteins,Journal of Proteome Research

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Comprehensive Proteomic Analysis of PGC7-Interacting Proteins
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-08-02 00:00:00 , DOI: 10.1021/acs.jproteome.6b00883
Hongliang Liu _{1,

2} , Lei Zhang _{1,

2} , Qing Wei _{1,

2} , Zhaopeng Shi _{1,

2} , Xiaoyan Shi _{2,

3} , Juan Du _{2,

4} , Chenyang Huang _{1,

2} , Yong Zhang _{1,

2} , Zekun Guo _{1,

2}

Affiliation

Primordial germ cell 7 (PGC7), a maternal factor essential for early development, plays a critical role in the regulation of DNA methylation, transcriptional repression, chromatin condensation, and cell division and the maintenance of cell pluripotentiality. Despite the fundamental roles of PGC7 in these cellular processes, only a few molecular and functional interactions of PGC7 have been reported. Here, a streptavidin–biotin affinity purification technique combined with LC–MS/MS was used to analyze potential proteins that interact with PGC7. In total, 291 potential PGC7-interacting proteins were identified. Through an in-depth bioinformatic analysis of potential interactors, we linked PGC7 to critical cellular processes including translation, RNA processing, cell cycle, and regulation of heterochromatin structure. To better understand the functional interactions of PGC7 with its potential interactors, we constructed a protein–protein interaction network using the STRING database. In addition, we discussed in detail the interactions between PGC7 and some of its newly validated partners. The identification of these potential interactors of PGC7 expands our knowledge on the PGC7 interactome and provides a valuable resource for understanding the diverse functions of this protein.

中文翻译：

PGC7相互作用蛋白的综合蛋白质组学分析。

原始生殖细胞7（PGC7）是早期发育必不可少的母体因子，在调节DNA甲基化，转录抑制，染色质浓缩和细胞分裂以及维持细胞多能性中起关键作用。尽管PGC7在这些细胞过程中起着基本作用，但仅报道了PGC7的一些分子和功能相互作用。在这里，链霉亲和素-生物素亲和纯化技术与LC-MS / MS结合使用，可分析与PGC7相互作用的潜在蛋白质。总共鉴定出291种潜在的PGC7相互作用蛋白。通过对潜在相互作用物的深入生物信息学分析，我们将PGC7与关键的细胞过程联系起来，包括翻译，RNA处理，细胞周期和异染色质结构的调节。为了更好地理解PGC7及其潜在相互作用物的功能相互作用，我们使用STRING数据库构建了一个蛋白质-蛋白质相互作用网络。此外，我们详细讨论了PGC7及其一些新验证的合作伙伴之间的相互作用。这些潜在的PGC7相互作用因子的鉴定扩展了我们对PGC7相互作用基因组的了解，并为了解该蛋白的多种功能提供了宝贵的资源。

更新日期：2017-08-03

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