Intracellular Uncaging of cGMP with Blue Light,ACS Chemical Neuroscience

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Intracellular Uncaging of cGMP with Blue Light
ACS Chemical Neuroscience ( IF 4.1 ) Pub Date : 2017-08-01 00:00:00 , DOI: 10.1021/acschemneuro.7b00237
Hitesh K. Agarwal ₁ , Shenyu Zhai ₂ , D. James Surmeier ₂ , Graham C. R. Ellis-Davies ₁

Affiliation

We have made a new caged cGMP that is photolyzed with blue light. Using our recently developed derivative of 7-diethylaminocourmarin (DEAC) called DEAC450, we synthesized coumarin phosphoester derivatives of cGMP with two negative charges appended to the DEAC450 moiety. DEAC450-cGMP is freely soluble in physiological buffer without the need for any organic cosolvents. With a photolysis quantum yield of 0.18 and an extinction coefficient of 43 000 M^–1 cm^–1 at 453 nm, DEAC450-cGMP is the most photosensitive caged cGMP made to date. In patch-clamped neurons in acutely isolated brain slices, blue light effectively uncaged cGMP from DEAC450 and facilitated activation of hyperpolarization and cyclic nucleotide gated cation (HCN) channels in cholinergic interneurons. Thus, DEAC450-cGMP has a unique set of optical and chemical properties that make it a useful addition to the optical arsenal available to neurobiologists.

中文翻译：

cGMP的胞内解笼与蓝光

我们制作了一个新的笼状cGMP，并用蓝光对其进行了光解。使用我们最近开发的称为DEAC450的7-二乙基氨基香豆素（DEAC）衍生物，我们合成了cGMP的香豆素磷酸酯衍生物，并在DEAC450部分附加了两个负电荷。DEAC450-cGMP可自由溶于生理缓冲液，而无需任何有机助溶剂。光解量子产率为0.18，消光系数为43000 M ^–1 cm ^–1在453 nm处，DEAC450-cGMP是迄今为止制造的最感光的笼状cGMP。在急性分离的脑切片中的膜片钳神经元中，蓝光有效地解封了DEAC450的cGMP，并促进了胆碱能中神经元的超极化和环状核苷酸门控阳离子（HCN）通道的激活。因此，DEAC450-cGMP具有独特的光学和化学性质，使其成为神经生物学家可用的光学武库的有用补充。

更新日期：2017-08-01

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