Measuring the Purity of Chitin with a Clean, Quantitative Solid-State NMR Method,ACS Sustainable Chemistry & Engineering

当前位置： X-MOL 学术 › ACS Sustain. Chem. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Measuring the Purity of Chitin with a Clean, Quantitative Solid-State NMR Method
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2017-07-31 00:00:00 , DOI: 10.1021/acssuschemeng.7b01589
Catherine King ₁ , Robin S. Stein ₁ , Julia L. Shamshina ₂ , Robin D. Rogers _{1,

2}

Affiliation

Chitin is a versatile biopolymer which can be extracted directly from biomass and used for the preparation of high value materials, but such materials often require high-purity chitin. However, complete removal of proteinaceous material from chitin, which is obtained from biomass sources, can be difficult to determine. Here we report a quick, nondestructive method to measure chitin content using solid-state multiCP ¹³C NMR, which requires only 105 min and allows for recovery of the sample. A calibration curve was constructed from spectra of mass-based mixtures of commercial chitin and bovine serum albumin protein. This allowed for the quantification of chitin content (and therefore purity) in both chitinous biomass and extracted chitin samples in a quick, clean, nondestructive manner that agreed with measurements made by the method of Black and Schwartz, within the error of each of the methods.

中文翻译：

使用干净的定量固态NMR方法测量甲壳质的纯度

几丁质是一种通用的生物聚合物，可以直接从生物质中提取并用于制备高价值的材料，但此类材料通常需要高纯度的几丁质。然而，难以确定从生物质来源获得的几丁质中蛋白质物质的完全去除。在这里，我们报告了一种使用固态multiCP ¹³测量甲壳质含量的快速，无损方法13 C NMR，仅需105分钟，即可回收样品。根据商品甲壳质和牛血清白蛋白的质量基混合物的光谱构建校正曲线。这样可以以快速，干净，无损的方式对几丁质生物质和提取的几丁质样品中的几丁质含量（以及纯度）进行定量，这与Black和Schwartz方法进行的测量结果一致，但每种方法均存在误差。

更新日期：2017-07-31

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南