Label-Free Monitoring of Single Molecule Immunoreaction with a Nanopipette,Analytical Chemistry

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Label-Free Monitoring of Single Molecule Immunoreaction with a Nanopipette
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-07-28 00:00:00 , DOI: 10.1021/acs.analchem.7b01921
Ru-Jia Yu ₁ , Yi-Lun Ying ₁ , Yong-Xu Hu ₁ , Rui Gao ₁ , Yi-Tao Long ₁

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The nanopipette has been employed for the single molecule analysis due to its advantage of easy fabrication and controllable diameter. Herein, we present that the single molecule immunoreaction could be monitored by using the quartz nanopipette through the discrimination of characteristic blockade current, which reflect the intrinsic character of the individual unlabeled protein molecules due to its heterogeneous motion in solution. Our methods show the ability to monitor the immunoreaction between single α-fetal protein (AFP) and its specific antibody in aqueous solution without any labeling. Our studies may open a new door to comprehensively understand the single molecule immunoreaction, which gain more insight into the molecular dynamic of elementary steps.

中文翻译：

用纳米移液器对单分子免疫反应进行无标签监测

纳米移液器由于其易于制造和可控制的直径的优势而被用于单分子分析。在这里，我们目前可以通过使用石英纳米移液管通过特征性阻断电流的辨别来监测单分子免疫反应，这反映了由于溶液中的异质运动而导致的各个未标记蛋白分子的内在特征。我们的方法显示了监控水溶液中单个α胎儿蛋白（AFP）及其特异性抗体之间的免疫反应的能力，而无需任何标记。我们的研究可能会为全面了解单分子免疫反应打开一扇新的大门，这将使您对基本步骤的分子动力学有更深入的了解。

更新日期：2017-07-30

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