A multiplex ultrasensitive electrochemiluminescence (ECL) immunoassay was developed for the simultaneous determination of two different tumor markers, cancer antigen 153 (CA 15-3) and cancer antigen 125 (CA 125) using polyamidoamine dendrimer-quantum dots (PAMAM-QDs) and PAMAM-sulfanilic acid-Ru(bpy)₃²⁺ as the signal probes and Fe₃O₄–SiO₂ as a magnetic bead. The [email protected] QDs and Ru(bpy)₃²⁺ at the presence of tripropyl amine (TPA) as coreactant generate ECL at an applied voltage of + 1.2 V (vs Ag/AgCl) in two different wavelengths 500 and 620 nm, respectively. Based on this strategy, the simultaneous detection of two tumor markers in single run carried out. This dual signal amplification technique was achieved by employing Fe₃O₄@SiO₂-dendrimer as immunosensing platform and PAMAM as the carrier for immobilizing [email protected] and Ru(bpy)₃²⁺ probes. Experimental results illustrated that the designed immunosensor can be used to sequentially detection of CA 125 and CA 15-3 markers with the wide linear ranges of 1 µU/mL to 1 U/mL and 0.1 mU/mL to 100 U/mL with very low detection limits of 0.1 µU/mL and 10 µU/mL, respectively. The application of the immunosensor for simultaneous detection of CA125 and CA15-3 in human serum samples was evaluated and the obtained results were found to be in acceptable agreement with the those obtained with an ELISA assay as reference method. The proposed ECL immunosensor can provide a simple, sensitive and reliable approach for the simultaneous detection of tumor markers in clinical samples.

开发了一种多重超灵敏电致发光（ECL）免疫测定法，可使用聚酰胺酰胺树状大分子量子点（PAMAM-QDs）和PAMAM同时测定两种不同的肿瘤标记物，即癌症抗原153（CA 15-3）和癌症抗原125（CA 125）。 -磺胺酸-Ru（bpy）₃ ²⁺作为信号探针，Fe ₃ O ₄ -SiO ₂作为磁珠。[受电子邮件保护的] QD和Ru（bpy）₃ ²⁺在三丙胺（TPA）作为共反应剂的存在下，当在+ 1.2 V（vs Ag / AgCl）施加的电压下，分别在两个不同的波长500和620 nm处产生ECL。基于此策略，可以在一次运行中同时检测两种肿瘤标记物。这种双重信号放大技术是通过使用Fe ₃ O ₄ @SiO _2-树状大分子作为免疫传感平台，并使用PAMAM作为固定化[email protected]和Ru（bpy）₃ ²⁺的载体来实现的。探针。实验结果表明，设计的免疫传感器可用于顺序检测CA 125和CA 15-3标记，线性标记范围从1 µU / mL到1 U / mL和0.1 mU / mL到100 U / mL，非常低检出限分别为0.1 µU / mL和10 µU / mL。评估了免疫传感器在人血清样品中同时检测CA125和CA15-3的应用，发现所获得的结果与以ELISA分析为参考方法所获得的结果是可以接受的。所提出的ECL免疫传感器可为同时检测临床样品中的肿瘤标志物提供简单，灵敏和可靠的方法。