Chamaecin (2-hydroxy-4-isopropylbenzaldehyde) was synthesized and tested for its tyrosinase inhibitory activity. It partially inhibits the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase with an IC(50) of 2.3 microM. The inhibition kinetics analyzed by Dixon plots found that chamaecin is a mixed type inhibitor. This inhibition may come in part from its ability to form a Schiff base with a primary amino group in the enzyme.

中文翻译：

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2-羟基-4-异丙基苯甲醛，一种有效的部分酪氨酸酶抑制剂。

合成了Chamaecin（2-羟基-4-异丙基苯甲醛）并测试了其酪氨酸酶抑制活性。它部分抑制了蘑菇酪氨酸酶催化的L-3,4-二羟基苯丙氨酸（L-DOPA）的氧化，IC（50）为2.3 microM。通过狄克逊图分析的抑制动力学发现，chamaecin是一种混合型抑制剂。这种抑制作用可能部分来自其形成酶中带有伯氨基的席夫碱的能力。