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Reversible Monolayer–Bilayer Transition in Supported Phospholipid LB Films under the Presence of Water: Morphological and Nanomechanical Behavior
Langmuir ( IF 3.7 ) Pub Date : 2017-07-20 00:00:00 , DOI: 10.1021/acs.langmuir.7b01268 Silvia Ruiz-Rincón , Alejandro González-Orive , Jesús M. de la Fuente 1, 2 , Pilar Cea 3
Langmuir ( IF 3.7 ) Pub Date : 2017-07-20 00:00:00 , DOI: 10.1021/acs.langmuir.7b01268 Silvia Ruiz-Rincón , Alejandro González-Orive , Jesús M. de la Fuente 1, 2 , Pilar Cea 3
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Mixed monolayer Langmuir–Blodgett (LB) films of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol (Chol) in the 1:1 ratio have been prepared onto solid mica substrates. Upon immersion in water or in an aqueous HEPES solution (pH 7.4) the monolayer LB films were spontaneously converted into well-organized bilayers leaving free mica areas. The process has been demonstrated to be reversible upon removal of the aqueous solution, resulting in remarkably free of defects monolayers that are homogeneously distributed onto the mica. In addition, the nanomechanical properties exhibited by the as-formed bilayers have been determined by means of AFM breakthrough force studies. The bilayers formed by immersion of the monolayer in an aqueous media exhibit nanomechanical properties and stability under compression analogous to those of DPPC:Chol supported bilayers obtained by other methods previously described in the literature. Consequently, the hydration of a monolayer LB film has been revealed as an easy method to produce well-ordered bilayers that mimic the cell membrane and that could be used as model cell membranes.
中文翻译：
在水存在下负载的磷脂LB薄膜中的可逆单层-双层转变：形态和纳米力学行为
1,2-二棕榈酰-sn混合单层Langmuir-Blodgett（LB）膜已将1：1比例的-甘油-3-磷酸胆碱（DPPC）和胆固醇（Chol）制备到固体云母基质上。浸入水或HEPES水溶液（pH 7.4）中后，单层LB膜自发转变为组织良好的双层，剩下自由的云母区域。已经证明该方法在除去水溶液后是可逆的，从而导致明显没有均匀分布在云母上的缺陷单层。另外，通过AFM突破力研究已经确定了所形成的双层所表现出的纳米机械性能。通过将单层浸入水性介质中形成的双层表现出类似于DPPC的纳米机械性能和在压缩下的稳定性：通过先前文献中所述的其他方法获得的Chol负载的双层。因此，单层LB膜的水合作用已被证明是一种易于生产的有序双层膜，可模仿细胞膜并可用作模型细胞膜的简便方法。
更新日期：2017-07-21
中文翻译：
在水存在下负载的磷脂LB薄膜中的可逆单层-双层转变：形态和纳米力学行为
1,2-二棕榈酰-sn混合单层Langmuir-Blodgett（LB）膜已将1：1比例的-甘油-3-磷酸胆碱（DPPC）和胆固醇（Chol）制备到固体云母基质上。浸入水或HEPES水溶液（pH 7.4）中后，单层LB膜自发转变为组织良好的双层，剩下自由的云母区域。已经证明该方法在除去水溶液后是可逆的，从而导致明显没有均匀分布在云母上的缺陷单层。另外，通过AFM突破力研究已经确定了所形成的双层所表现出的纳米机械性能。通过将单层浸入水性介质中形成的双层表现出类似于DPPC的纳米机械性能和在压缩下的稳定性：通过先前文献中所述的其他方法获得的Chol负载的双层。因此，单层LB膜的水合作用已被证明是一种易于生产的有序双层膜，可模仿细胞膜并可用作模型细胞膜的简便方法。
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