Oleoyl-Chitosan-Based Nanofiber Mats Impregnated with Amniotic Membrane Derived Stem Cells for Accelerated Full-Thickness Excisional Wound Healing,ACS Biomaterials Science & Engineering

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Oleoyl-Chitosan-Based Nanofiber Mats Impregnated with Amniotic Membrane Derived Stem Cells for Accelerated Full-Thickness Excisional Wound Healing
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-07-19 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00189
Sayanti Datta , Arun Prabhu Rameshbabu , Kamakshi Bankoti , Priti Prasanna Maity , Dipankar Das ₁ , Sagar Pal ₁ , Sabyasachi Roy ₂ , Ramkrishna Sen , Santanu Dhara

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Wound healing management is a major challenge for critical full-thickness skin wounds. Development of nanofibrous scaffolds with tunable wettability, degradation, and biocompatibility are highly desirable. Herein, we demonstrated synthesis of oleoyl chitosan (OC) by grafting monounsaturated fatty acid residue, C₁₈ oleoyl chain, to the backbone of chitosan molecule and blending with gelatin to form the nanofiber mats. The physicochemical properties of the nanofiber mats revealed mechanical strength, moderate surface wettability, and suitable degradation rate. The nanofibrous mats showed excellent in vitro cytocompatibility with human amniotic membrane-derived stem cells (HAMSCs) in terms of enhanced adhesion and proliferation owing to biomimetic nanoarchitecture and chemical cues. Furthermore, the fabricated nanofiber was implanted with and without preseeded HAMSCs in the full-thickness wound to evaluate the skin wound healing efficacy in a rat model. Histological and immunohistochemical studies were conducted to evaluate the plausible changes of tissue architecture and expression of molecular markers involved in wound healing process. Both acellular and HAMSCs incorporated cellular nanofibers promoted wound contraction remarkably with superior skin tissue regeneration in terms of enhanced collagen synthesis, re-epithelialization and initiation of epithelial cells stratification compared to control group.

中文翻译：

羊膜衍生干细胞浸渍油基壳聚糖为基础的纳米纤维垫，用于加速全层切除伤口愈合。

伤口愈合管理是关键的全层皮肤伤口的主要挑战。具有可调节的润湿性，降解性和生物相容性的纳米纤维支架的开发是非常需要的。本文中，我们通过接枝单不饱和脂肪酸残基C ₁₈证明了油酰壳聚糖（OC）的合成油酰链，连接到壳聚糖分子的骨架，并与明胶混合形成纳米纤维垫。纳米纤维垫的物理化学性质显示出机械强度，适度的表面润湿性和合适的降解速率。纳米纤维垫与人类羊膜衍生干细胞（HAMSCs）表现出优异的体外细胞相容性，这是由于仿生纳米结构和化学提示而增强的附着力和增殖。此外，将所制备的纳米纤维在全厚度伤口中植入或不植入预先植入的HAMSC，以评估大鼠模型中皮肤伤口的愈合功效。进行了组织学和免疫组织化学研究，以评估组织结构的合理变化和伤口愈合过程中涉及的分子标志物的表达。

更新日期：2017-07-20

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