PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis,Chemistry - A European Journal

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PCR Incorporation of Polyoxometalate Modified Deoxynucleotide Triphosphates and Their Application in Molecular Electrochemical Sensing of Yersinia pestis
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2017-07-18 06:15:37 , DOI: 10.1002/chem.201701295
Mayreli Ortiz ₁ , Ahmed M. Debela ₂ , Marketa Svobodova ₁ , Serge Thorimbert ₂ , Denis Lesage ₂ , Richard B. Cole ₂ , Bernold Hasenknopf ₂ , Ciara K. O'Sullivan _{1,

3}

Affiliation

Redox-labeled nucleotides are of increasing interest for the fabrication of next generation molecular tools and should meet requirements of being thermally stable, sensitive, and compatible with polymerase-mediated incorporation while also being electrochemically discriminable. The synthesis and characterization of Keggin and Dawson polyoxometalate–deoxynucleotide (POM–dNTP) bioconjugates linked through 7-deaza-modified purines is described. The modified POM–dNTPs were used for polymerase-based amplification of a DNA sequence specific for Yersinia pestis and the amplified DNA detected using an electrochemical DNA sensor. This highlights the potential of polyoxometalates as thermally stable, sensitive and polymerase-compatible redox labels for exploitation in bioanalytical applications.

中文翻译：

多金属氧酸盐修饰的脱氧核苷酸三磷酸的PCR结合及其在鼠疫耶尔森菌的分子电化学传感中的应用

氧化还原标记的核苷酸在制造下一代分子工具中越来越受到关注，并应满足热稳定，敏感并与聚合酶介导的掺入兼容的要求，同时也可通过电化学方法加以区分。描述了通过7-脱氮基修饰的嘌呤连接的Keggin和Dawson多金属氧酸盐-脱氧核苷酸（POM-dNTP）生物缀合物的合成和表征。修饰的POM-dNTPs用于基于聚合酶的鼠疫耶尔森氏菌特异性DNA序列扩增，并使用电化学DNA传感器检测扩增的DNA。这突出了多金属氧酸盐作为热稳定，敏感和聚合酶兼容的氧化还原标记物的潜力，可用于生物分析应用。

更新日期：2017-07-19

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