3'-磷酸腺苷5'-磷酸硫酸盐转运蛋白的分子克隆和鉴定。,Journal of Biological Chemistry

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3'-磷酸腺苷5'-磷酸硫酸盐转运蛋白的分子克隆和鉴定。
Journal of Biological Chemistry ( IF 4.0 ) Pub Date : 2003 Jul 11 , DOI: 10.1074/jbc.m302439200
Shin Kamiyama , Takeshi Suda , Ryu Ueda , Masayuki Suzuki , Reiko Okubo , Norihiro Kikuchi , Yasunori Chiba , Satoshi Goto , Hidenao Toyoda , Kaoru Saigo , Masahiko Watanabe , Hisashi Narimatsu , Yoshifumi Jigami , Shoko Nishihara

硫酸核苷酸，即3'-磷酸腺苷5'-磷酸磷酸酯（PAPS），是用于硫酸盐化的通用硫酰基供体。尽管高尔基体膜中存在特定的PAPS转运蛋白，但尚无研究报道相应的基因。我们已经鉴定出一种编码PAPS转运蛋白的新型人类基因，我们将其命名为PAPST1，与果蝇果蝇直系同源物回旋（slllom）。PAPST1的氨基酸序列（432个氨基酸）与SLL（465个氨基酸）具有48.1％的同一性，亲水性分析预测这两个蛋白是III型跨膜蛋白。PAPST1在SW480细胞中的瞬时表达表明在高尔基体膜中存在亚细胞定位。PAPST1和SLL在酿酒酵母中的表达显着增加了PAPS向高尔基体膜部分的转运。在人体组织中 PAPST1在胎盘和胰腺中高表达，在结肠和心脏中含量较低。用GAL4-UAS系统产生的sll RNA干扰蝇显示，PAPS转运蛋白对生存力至关重要。众所周知，与PAPS合成有关的某些基因的突变是人类遗传性疾病的原因。我们的发现为PAPS转运和翻译后硫酸化的重要性提供了见解。

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Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter.

Nucleotide sulfate, namely 3'-phosphoadenosine 5'-phosphosulfate (PAPS), is a universal sulfuryl donor for sulfation. Although a specific PAPS transporter is present in Golgi membrane, no study has reported the corresponding gene. We have identified a novel human gene encoding a PAPS transporter, which we have named PAPST1, and the Drosophila melanogaster ortholog, slalom (sll). The amino acid sequence of PAPST1 (432 amino acids) exhibited 48.1% identity with SLL (465 amino acids), and hydropathy analysis predicted the two to be type III transmembrane proteins. The transient expression of PAPST1 in SW480 cells showed a subcellular localization in Golgi membrane. The expression of PAPST1 and SLL in yeast Saccharomyces cerevisiae significantly increased the transport of PAPS into the Golgi membrane fraction. In human tissues, PAPST1 is highly expressed in the placenta and pancreas and present at lower levels in the colon and heart. An RNA interference fly of sll produced with a GAL4-UAS system revealed that the PAPS transporter is essential for viability. It is well known that mutations of some genes related to PAPS synthesis are responsible for human inherited disorders. Our findings provide insights into the significance of PAPS transport and post-translational sulfation.

更新日期：2017-01-31

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