Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

中文翻译：

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谷胱甘肽的实时定量成像。

谷胱甘肽在生物过程中起着许多重要的作用。然而，活细胞中谷胱甘肽浓度的动态变化仍是未知之数。在这里，我们报告了一种基于可逆反应的荧光探针，称为RealThiol（RT），它可以定量监测活细胞中的实时谷胱甘肽动力学。使用逆转录酶，我们观察到在肥大症期间激活的神经元的抗氧化能力增强和动态谷胱甘肽变化。因此，RT是一种多功能工具，可用于基于共聚焦显微镜和流式细胞仪的单细胞中谷胱甘肽水平的高通量定量分析。我们设想，这种新的谷胱甘肽探针将为研究谷胱甘肽动力学和运输提供机会，并扩大我们对谷胱甘肽在活细胞中的生理和病理作用的理解。