Co-regulation between adipocytes and supporting vasculature is considered an important process in adipose tissue generation. The objective of this study was to evaluate the mechanical and biological effects of a distraction technique on adipose tissue formation and maintenance. Based on the hypothesis that fat flaps gradually receding from each other can develop an adipose tissue construct, perforated polycarbonate syringe-shaped chambers were implanted in a rabbit model. Latency (1 week) and distraction (3 weeks) periods were followed by a consolidation period in the experimental groups (4, 8, and 12 weeks). In the distraction group, the volume of fat pad gradually increased up to 16 weeks. A transition zone was observed at 8 weeks, indicating the initiation of tissue generation. Histomorphologic analysis showed adipose and collagen connective tissue at 8 weeks. At 16 weeks, the relative composition was altered significantly. Adipose components occupied most of the tissue, and connective tissue was reduced. Blood vessels with endothelial lining were noted adjacent to adipocyte clusters, as well as in inter-adipocyte areas. The vessels had increased in number and were evenly distributed by 16 weeks. Our distraction technique produced more balanced adipose tissue generation than a non-distraction method, with co-development of adipose and vascular tissues.

中文翻译：

---

利用脂肪瓣分散技术形成脂肪组织。

脂肪细胞和支持性脉管系统之间的共同调节被认为是脂肪组织生成的重要过程。这项研究的目的是评估分散技术对脂肪组织形成和维持的机械和生物学影响。基于脂肪皮瓣逐渐彼此退缩可以形成脂肪组织构造的假说，在兔子模型中植入了多孔聚碳酸酯注射器形腔室。在实验组中，潜伏期（1周）和注意力分散（3周）期之后是巩固期（4、8和12周）。在分心组中，脂肪垫的体积逐渐增加，直至16周。在第8周观察到过渡区，表明组织生成的开始。组织形态学分析显示8周时脂肪和胶原结缔组织。在16周时，相对组成发生了显着变化。脂肪成分占据了大部分组织，结缔组织减少了。带有血管内皮衬层的血管在脂肪细胞簇附近以及在脂肪细胞间区域被发现。船只数量增加，并在16周内平均分配。与非分散方法相比，与脂肪和血管组织共同发展，我们的分散技术可产生更均衡的脂肪组织生成。以及脂肪细胞间区域。船只数量增加，并在16周内平均分配。与非分散方法相比，与脂肪和血管组织共同发展，我们的分散技术可产生更均衡的脂肪组织生成。以及脂肪细胞间区域。船只数量增加，并在16周内平均分配。与非分散方法相比，与脂肪和血管组织共同发展，我们的分散技术可产生更均衡的脂肪组织生成。